Transcriptional activation and translocation of ancient NOS during immune response
文献类型：期刊论文
| 作者 | Jiang, Qiufen1,2; Liu, Zhaoqun1,2 ; Zhou, Zhi1; Wang, Lingling1,3; Wang, Leilei1,2; Yue, Feng1,2; Wang, Jingjing1,2; Wang, Hao1; Song, Linsheng3
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| 刊名 | FASEB JOURNAL
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 30期号:10页码:3527-3540 |
| 关键词 | nitric oxide synthase NF-kappa B STAT |
| 英文摘要 | NOS is the key component of the NO system, which plays an indispensable role in many physiologic and immunologic processes; however, the process that underlies the activation of ancient NOSs and their functions remains unclear. Expression of Crassostrea gigas NOS (CgNOS) mRNA in hemocytes was examined after stimulating oysters with LPS and TNF-alpha Expression level of CgNOS mRNA was increased significantly, by 2.61-fold (P < 0.05), at 24 h poststimulation. A positive CgNOS signal was detected via immunoprecipitation, and only one protein was detected in oyster hemocytes. Shifting and supershifting bands were observed in EMSAs between the CgNOS promoter and the transcription factors CgNF-kappa B1 and Cg-signal transducer and activator of transcription (STAT). CgNF-kappa B1 was detected in the nucleus only at 12 h, whereas CgSTAT was observed in the cytoplasm and nucleus at 12 and 24 h. Expression levels of tyrosine-protein kinase receptor Tie-1, phosphatidylinositide phosphatase SAC2, phosphatidylinositol-4-phosphate 5-kinase type-1, diacylglycerol kinase , LPS-induced TNF-alpha factor-like protein, cAMP-dependent transcription factor-2, NF-kappa B1, and STAT6 were significantly elevated in a transcriptome analysis after 12 h of LPS and TNF-alpha stimulation. An immunoreactive CgNOS signal was observed in both the cell membrane and cytoplasm at 12 h, whereas it was mainly localized to the cytoplasm at 24 h post-LPS and -TNF-alpha stimulation. These findings revealed that CgNOS could be transcriptionally activated by CgNF-kappa B1 and CgSTAT via the PI3K-Akt pathway, similar to what occurs for iNOS, but CgNOS translocated to the cytoplasm, similar to neuronal NOS, to modulate downstream signals during an immune defense. These results collectively provide crucial knowledge about the evolution of NOS structure and function. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000384329800022 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/135887]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Qiufen,Liu, Zhaoqun,Zhou, Zhi,et al. Transcriptional activation and translocation of ancient NOS during immune response[J]. FASEB JOURNAL,2016,30(10):3527-3540. |
| APA | Jiang, Qiufen.,Liu, Zhaoqun.,Zhou, Zhi.,Wang, Lingling.,Wang, Leilei.,...&Song, Linsheng.(2016).Transcriptional activation and translocation of ancient NOS during immune response.FASEB JOURNAL,30(10),3527-3540. |
| MLA | Jiang, Qiufen,et al."Transcriptional activation and translocation of ancient NOS during immune response".FASEB JOURNAL 30.10(2016):3527-3540. |
入库方式： OAI收割
来源：海洋研究所
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