Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC
文献类型：期刊论文
| 作者 | Luan, Guangxiang1,3; Wang, Honglun1,2; Lv, Huanhuan1,3; Hu, Na1; Suo, Yourui1; Wang, Xiaoyan1 |
| 刊名 | chromatographia
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 79期号:13-14页码:823-831 |
| 关键词 | HSCCC Semi-preparative RPLC Caragana korshinskii Kom Flavone glucosides Lignan Separation |
| 英文摘要 | a method of using high-speed counter-current chromatography (hsccc) and semi-preparative reversed-phase liquid chromatography (semi-preparative rplc) to preparatively separate flavone glucosides and lignan from the crude extracts of caragana korshinskii has been established for the first time in this study. five flavone glucosides, including 9 mg of kaempferol 3-o-{beta-d-glucopyranosyl(1 -> 2)-[alpha-l-rhamonopyranosyl(1 -> 6)]-beta-d-galactopyranoside}, 21 mg of kaempferol 3-o-alpha-l-rhamnopyranosyl(1 -> 6)-beta-d-galactopyranoside-7-o-alpha-l-rhamnopyranoside, 34 mg of kaempferol 3-o-beta-d-galactopyranoside-7-o-alpha-l-rhamnopyranoside, 27 mg of kaempferol 3-o-beta-d-glucopyranosyl-7-o-alpha-l-rhamnopyranoside, and 14 mg of calycosin 7-o-beta-d-glucopyranoside, and one lignan, 16 mg of alangilignoside b, were successfully isolated from 1.8 g of the crude sample through the combination of hsccc with a two-phase solvent system composed of ethyl acetate-n-butanol-0.5 % formic acid (4:1:5, v/v/v) and semi-preparative rplc with a mobile phase of methanol-water (20:80, v/v). the purities of the six compounds are all over 95 % as determined by hplc and the structures are confirmed by the analysis of uv, h-1-nmr, and c-13-nmr and compared with published data. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | counter-current chromatography ; glycosides ; isoflavones ; chinensis ; shift ; seeds ; hplc |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000379157500003 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6475]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Huzhou Plateau Biol Resource Ctr Innovat, Huzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luan, Guangxiang,Wang, Honglun,Lv, Huanhuan,et al. Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC[J]. chromatographia,2016,79(13-14):823-831. |
| APA | Luan, Guangxiang,Wang, Honglun,Lv, Huanhuan,Hu, Na,Suo, Yourui,&Wang, Xiaoyan.(2016).Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC.chromatographia,79(13-14),823-831. |
| MLA | Luan, Guangxiang,et al."Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC".chromatographia 79.13-14(2016):823-831. |
入库方式： OAI收割
来源：西北高原生物研究所
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