Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography
文献类型：期刊论文
| 作者 | Wang, Yan-Ming1,2,4; Zhao, Jian-Qiang3,4; Yang, Jun-Li1,2; Tao, Yan-Duo3; Mei, Li-Juan3; Shi, Yan-Ping1,2 |
| 刊名 | journal of agricultural and food chemistry
 |
| 出版日期 | 2016-06-22 |
| 卷号 | 64期号:24页码:4950-4957 |
| 关键词 | Asterothamnus centrali-asiaticus triterpenoid saponins 2D RPLC/HILIC cytotoxicity |
| 英文摘要 | asterothamnus centrali-asiaticus, a kind of characteristic shrub abundant in grassland and desert areas, has been used as forage fodder for camels and goats in central asia, and this plant also plays a critical role in the maintenance of desert grassland ecosystems as a result of its tolerance to poor soils and sand burial. however, its chemical composition has been rarely reported. in this study, phytochemical investigation of this pasturage was performed and three new triterpenoid saponins (1-3) were isolated together with nine known triterpenoid saponins (4-12) using preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography (2d rplc/hilic). their structures were elucidated via diverse spectroscopic analyses, including infrared (ir) spectrometry, high-resolution electrospray ionization mass spectrometry (hr-esims), and one-dimensional (1d) and 2d nuclear magnetic resonance (nmr). all isolated triterpenoid saponins (1-12) were reported from this genus for the first time, and they were further evaluated for their cytotoxicity against four cancer cell lines (a549, hepg2, mgc-803, and mfc), which indicated that compound 11 showed potent cytotoxicity against the hepg2 cell line, with an ic50 value of 6.85 mu g/ml. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | agriculture, multidisciplinary ; chemistry, applied ; food science & technology |
| 研究领域[WOS] | agriculture ; chemistry ; food science & technology |
| 关键词[WOS] | hydrophilic interaction chromatography ; triterpenoid saponins ; platycodon-grandiflorum ; aster-auriculatus ; purification ; separation ; bufadienolides ; ginsenosides ; column ; roots |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000378584300016 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6481]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yan-Ming,Zhao, Jian-Qiang,Yang, Jun-Li,et al. Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography[J]. journal of agricultural and food chemistry,2016,64(24):4950-4957. |
| APA | Wang, Yan-Ming,Zhao, Jian-Qiang,Yang, Jun-Li,Tao, Yan-Duo,Mei, Li-Juan,&Shi, Yan-Ping.(2016).Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography.journal of agricultural and food chemistry,64(24),4950-4957. |
| MLA | Wang, Yan-Ming,et al."Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography".journal of agricultural and food chemistry 64.24(2016):4950-4957. |
入库方式： OAI收割
来源：西北高原生物研究所
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