Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum
文献类型：期刊论文
| 作者 | Chen, Tao1; Liu, Yong-Ling1,2; Chen, Chen1; Zou, Deng-Lang1,2; You, Jin-Mao1; Sun, Jing1; Li, Yu-Lin1 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2014-04-15 |
| 卷号 | 957页码:90-95 |
| 关键词 | Rheum tanguticum Anthraquinone Glycoside Stilbene glycoside Macroporous resin High-speed counter-current chromatography |
| 通讯作者 | sun, j (reprint author), chinese acad sci, northwest inst plateau biol, key lab tibetan med res, xining 810001, peoples r china. |
| 英文摘要 | in this paper, an efficient method was successfully established by the combination of macroporous resin (mr) and high-speed counter-current chromatography (hsccc) for rapid enrichment and separation of aloe-emodin 8-o-beta-d-glucoside, emodin 1-o-beta-d-glucoside, emodin 8-o-beta-d-glucoside and piceatannol 4'-o-beta-d-(6 ''-o-gallate)-glucoside. six kinds of macroporous resins were investigated in the first step and x-5 macroporous resin was selected for the enrichment of the target compounds. the recoveries of the target compounds reached 89.0, 85.9, 82.3 and 84.9% respectively after 40% ethanol elution. in the second step, the target compounds were separated by hsccc with a two-phase solvent system composed of chloroform/ethyl acetate/methanol/water (8:1:6:5, v/v). the established method will be helpful for further characterization and utilization of rheum tanguticum. the results demonstrate that mr coupled with hsccc is a powerful technique for separation of bioactive compounds from natural products. (c) 2014 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | maxim. ex balf. ; chemical-constituents ; parthenocissus-tricuspidata ; polygonum-multiflorum ; preparative isolation ; purification ; rhubarb ; identification ; requirements ; derivatives |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350599300014 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6585]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Tao,Liu, Yong-Ling,Chen, Chen,et al. Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2014,957:90-95. |
| APA | Chen, Tao.,Liu, Yong-Ling.,Chen, Chen.,Zou, Deng-Lang.,You, Jin-Mao.,...&Li, Yu-Lin.(2014).Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum.journal of chromatography b-analytical technologies in the biomedical and life sciences,957,90-95. |
| MLA | Chen, Tao,et al."Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum".journal of chromatography b-analytical technologies in the biomedical and life sciences 957(2014):90-95. |
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来源：西北高原生物研究所
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