Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization
文献类型：期刊论文
| 作者 | Lv, Tao1,2; Zhao, Xian-En1,2; Zhu, Shuyun1,2; Ji, Zhongyin1,3; Chen, Guang1,2; Sun, Zhiwei1,2; Song, Cuihua1,2; You, Jinmao1,2,3; Suo, Yourui3 |
| 刊名 | chromatographia
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 77期号:23-24页码:1653-1660 |
| 关键词 | Column liquid chromatography Ultrasonic-assisted dispersive liquid-liquid microextraction derivatization Fluorescence detection Brassinolide Plant matrix |
| 通讯作者 | zhao, xe (reprint author), qufu normal univ, coll chem & chem engn, shandong prov key lab life organ anal, qufu 273165, shandong, peoples r china. |
| 英文摘要 | a novel hyphenated technique based on ultrasonic-assisted dispersive liquid-liquid microextraction (ua-dllme) coupled with derivatization has been established for the determination of brassinolide (bl, a representative of brassinosteroids) by hplc fluorescence detection. 9-phenanthreneboronic acid is used as labeling reagent of bl. ua-dllme parameters containing type and volume of extraction and disperser solvent, ph and ultrasonication time are optimized. derivatization parameters are optimized included amount of 9-phenanthreneboronic acid, volume ratio of pyridine, derivatization time and temperature. under optimal conditions, quantitative linear range of bl is 50-1,000 ng l-1 and excellent linear response is observed with correlation coefficient of 0.9996. limit of detection and limit of quantification are calculated as 8.0 and 25.0 ng l-1, respectively. rsds of retention time and peak area are in the range of 0.68-0.97 % and 4.61-6.54 % for intra-day precision, 1.32-1.94 % and 7.28-9.75 % for inter-day precision, respectively. accuracy is satisfactory in the range of 82.3-125.1 %. rsds' values of repeatability are in the range of 0.82-1.79 and 3.95-8.53 % for retention time and peak area, respectively. enrichment factor for bl is 189. the results of recovery and matrix effect are in the range of 82.0-108.6 and 90.0-115.3 %, respectively. the proposed method has been applied for the determination of bl in arabidopsis thaliana, daucus carota and brassica campestris l. leaves with much higher sensitivity than many other methods. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | polymer monolith microextraction ; chromatography-mass-spectrometry ; sensitive analysis ; brassinosteroids ; pollen ; plants ; kuntze ; water |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345382600010 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6589]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Tao,Zhao, Xian-En,Zhu, Shuyun,et al. Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization[J]. chromatographia,2014,77(23-24):1653-1660. |
| APA | Lv, Tao.,Zhao, Xian-En.,Zhu, Shuyun.,Ji, Zhongyin.,Chen, Guang.,...&Suo, Yourui.(2014).Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization.chromatographia,77(23-24),1653-1660. |
| MLA | Lv, Tao,et al."Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization".chromatographia 77.23-24(2014):1653-1660. |
入库方式： OAI收割
来源：西北高原生物研究所
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