中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis

文献类型:期刊论文

作者Yang, Renming1; Wang, Honglun1; Li, Gang2; Suo, Yourui1; You, Jinmao3
刊名asian journal of chemistry
出版日期2014-08-01
卷号26期号:15页码:4830-4834
关键词Fenugreek Flavonoids Response surface methodology Ultrasound-assisted extraction Quantitative analysis
通讯作者wang, hl (reprint author), chinese acad sci, northwest inst plateau biol, key lab tibetan med res, xining 810001, peoples r china.
英文摘要in this study, a central composite design based on response surface methodology was used to optimize ultrasonic-assistant extraction of flavonoids from trigonella foenum-graecum l. the optimal conditions to obtain the highest yield of total flavonoids were as follows: ultrasonic time of 80 min, temperature of 48 degrees c and liquid to solid ratio of 20 g/ml, where 1.03 % yield was predicted. after extraction process optimization, a method for the analysis of flavonoids in trigonella foenum-graecum l. by hplc-dad-ms was developed. the lod and loq of these 4 compounds range from 50.94 to 70.31 ng/ml and 179.63 to 208.85 ng/ml, respectively. the established hplc-dad-ms method was proven to be useful for the quantitative and qualitative analysis of flavonoid constituents in fenugreek seed. this is the first report on preparation, identification and determination of flavonoids in fenugreek seed by different method and which would provide a basis for medicinal and edible use of fenugreek.
WOS标题词science & technology ; physical sciences
类目[WOS]chemistry, multidisciplinary
研究领域[WOS]chemistry
关键词[WOS]trigonella-foenum-graecum ; seeds ; glycosides ; phenolics
收录类别SCI
语种英语
WOS记录号WOS:000343766300062
源URL[http://ir.nwipb.ac.cn/handle/363003/6597]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China
2.Yantai Univ, Life Sch, Yantai 264005, Peoples R China
3.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Yang, Renming,Wang, Honglun,Li, Gang,et al. Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis[J]. asian journal of chemistry,2014,26(15):4830-4834.
APA Yang, Renming,Wang, Honglun,Li, Gang,Suo, Yourui,&You, Jinmao.(2014).Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis.asian journal of chemistry,26(15),4830-4834.
MLA Yang, Renming,et al."Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis".asian journal of chemistry 26.15(2014):4830-4834.

入库方式: OAI收割

来源:西北高原生物研究所

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