利用聚乙二醇(PEG)-硫酸铵双水相体系对鱼腥草总黄酮进行萃取分离。通过单因素试验分别考察了PEG分子量、两相质量比、萃取温度、萃取p H对分离提取鱼腥草中总黄酮的影响,并用正交试验优化了萃取工艺。经优化后,鱼腥草总黄酮在以m PEG800:m(NH4)2SO4=2.0:2.0为双水相体系,萃取p H为3.0,水浴温度为50℃时,萃取率可高达98.96%。本方法简单高效,绿色环保,可为鱼腥草总黄酮的高效利用提供参考。 更多还原
 

To separate the total flavonoids from Houttuyniae cordata Thunb by aueous two-phase
systems composed of polyethylene glycol (PEG) and phosphate salts, an orthogonal experimental design method was applied to evaluate the influence of the average molecular mass of PEG, mass ratio of the two phases, temperature and pH value on total flavonoids extraction. The extraction rate was achieved 98.96 % under the optimal conditions: mPEG400: m(NH4)2SO4=2.0: 2.0, temperature was 50 ℃, pH was 3.0. The result indicated that, the aqueous two-phase extraction was simple, high quality and environment friendly, and also can apply some useful information for the efficientuse of the total flavonoids in Houttuyniae cordata Thunb.