Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin
文献类型：期刊论文
| 作者 | Hu, Bodan; Du, Tao; Li, Chang; Luo, Sukun; Liu, Yalan; Huang, Xin; Hu, Qinxue |
| 刊名 | JOURNAL OF GENERAL VIROLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 96页码:3660-3666 |
| 关键词 | HIV-1 GP120 INACTIVATING PROTEIN GLYCOSYLATION SITES GLYCOPROTEIN GP120 GLYCAN DELETIONS PLANT-LECTINS MANNOSE RESISTANCE TRANSMISSION INFECTIONS |
| 产权排序 | 3 |
| 通讯作者 | Hu, QX (reprint author), Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China. ; Hu, QX (reprint author), Univ London, Inst Infect & Immun, London SW17 0RE, England. |
| 合作状况 | 国际 |
| 英文摘要 | Human immunodeficiency virus type 1 (HIV-1) transmission often results from infection by a single transmitted/founder (T/F) virus. Here, we investigated the sensitivity of T/F HIV-1 envelope glycoproteins (Envs) to microbicide candidate carbohydrate-binding agents (CBAs) griffithsin (GRFT), cyanovirin-N (CV-N) and Galanthus nivalis agglutinin (GNA), showing that T/F Envs demonstrated different sensitivity to CBAs, with IC50 values ranging from 0.006 +/- 0.0003 to > 10 nM for GRFT, from 0.6 +/- 0.2 to 28.9 +/- 2.9 nM for CV-N and from 1.3 +/- 0.2 to > 500 nM for GNA. We further revealed that deglycosylation at position 295 or 448 decreased the sensitivity of T/F Env to GRFT, and at 339 to both CV-N and GNA. Mutation of all the three glcyans rendered a CBA-sensitive T/F Env largely resistant to GRFT, indicating that the sensitivity of T/F Env to GRFT is mainly determined by glycans at 295, 339 and 448. Our study identified specific T/F Env residues associated with CBA sensitivity. |
| 学科主题 | Biotechnology & Applied Microbiology; Virology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/27554]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Hu, Bodan,Du, Tao,Li, Chang,et al. Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin[J]. JOURNAL OF GENERAL VIROLOGY,2015,96:3660-3666. |
| APA | Hu, Bodan.,Du, Tao.,Li, Chang.,Luo, Sukun.,Liu, Yalan.,...&Hu, Qinxue.(2015).Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin.JOURNAL OF GENERAL VIROLOGY,96,3660-3666. |
| MLA | Hu, Bodan,et al."Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin".JOURNAL OF GENERAL VIROLOGY 96(2015):3660-3666. |
入库方式： OAI收割
来源：成都生物研究所
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