A Rapid Study of Botanical Drug-Drug Interaction with Protein by Re-ligand Fishing using Human Serum Albumin-Functionalized Magnetic Nanoparticles
文献类型：期刊论文
| 作者 | Qing, Lin-Sen; Xue, Ying; Ding, Li-Sheng; Liu, Yi-Ming; Liang, Jian; Liao, Xun |
| 刊名 | NATURAL PRODUCT COMMUNICATIONS
 |
| 出版日期 | 2015 |
| 卷号 | 10期号:12页码:2161-2163 |
| 关键词 | IONIZATION MASS-SPECTROMETRY IN-VITRO BINDING |
| 产权排序 | 1 |
| 通讯作者 | Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China. |
| 合作状况 | 国际 |
| 英文摘要 | A great many active constituents of botanical drugs bind to human serum albumin (HSA) reversibly with a dynamic balance between the free- and bound-forms in blood. The curative or side effect of a drug depends on its free-form level, which is always influenced by other drugs, combined dosed or multi-constituents of botanical drugs. This paper presented a rapid and convenient methodology to investigate the drug-drug interactions with HSA. The interaction of two steroidal saponins, dioscin and pseudo-protodioscin, from a botanical drug was studied for their equilibrium time and equilibrium amount by re-ligand fishing using HSA functionalized magnetic nanoparticles. A clear competitive situation was obtained by this method. The equilibrium was reached soon about 15 s at a ratio of 0.44: 1. Furthermore, the interaction of pseudo-protodioscin to total steroidal saponins from DAXXK was also studied. The operation procedures of this method were faster and more convenient compared with other methods reported. |
| 学科主题 | Pharmacology & Pharmacy; Food Science & Technology |
| 类目[WOS] | Chemistry, Medicinal ; Food Science & Technology |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/27557]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Qing, Lin-Sen,Xue, Ying,Ding, Li-Sheng,et al. A Rapid Study of Botanical Drug-Drug Interaction with Protein by Re-ligand Fishing using Human Serum Albumin-Functionalized Magnetic Nanoparticles[J]. NATURAL PRODUCT COMMUNICATIONS,2015,10(12):2161-2163. |
| APA | Qing, Lin-Sen,Xue, Ying,Ding, Li-Sheng,Liu, Yi-Ming,Liang, Jian,&Liao, Xun.(2015).A Rapid Study of Botanical Drug-Drug Interaction with Protein by Re-ligand Fishing using Human Serum Albumin-Functionalized Magnetic Nanoparticles.NATURAL PRODUCT COMMUNICATIONS,10(12),2161-2163. |
| MLA | Qing, Lin-Sen,et al."A Rapid Study of Botanical Drug-Drug Interaction with Protein by Re-ligand Fishing using Human Serum Albumin-Functionalized Magnetic Nanoparticles".NATURAL PRODUCT COMMUNICATIONS 10.12(2015):2161-2163. |
入库方式： OAI收割
来源：成都生物研究所
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