Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin
文献类型：期刊论文
| 作者 | Zhao, Yongyun; Chen, Gangyi; Yuan, Yi; Li, Na; Dong, Juan; Huang, Xin; Cui, Xin; Tang, Zhuo |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015 |
| 卷号 | 5页码:- |
| 关键词 | IN-VITRO SELECTION U BASE-PAIRS ARTIFICIAL RIBONUCLEASES HAMMERHEAD RIBOZYME CLEAVAGE SITE DEOXYRIBOZYMES MIMICS |
| 产权排序 | 1 |
| 通讯作者 | Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China. |
| 合作状况 | 国内 |
| 英文摘要 | Constant efforts have been made to develop new method to realize sequence-specific RNA degradation, which could cause inhibition of the expression of targeted gene. Herein, by using an unmodified short DNA oligonucleotide for sequence recognition and endogenic small molecue, vitamin B2 (riboflavin) as photosensitizer, we report a simple strategy to realize the sequence-specific photocleavage of targeted RNA. The DNA strand is complimentary to the target sequence to form DNA/RNA duplex containing a G.U wobble in the middle. The cleavage reaction goes through oxidative elimination mechanism at the nucleoside downstream of U of the G.U wobble in duplex to obtain unnatural RNA terminal, and the whole process is under tight control by using light as switch, which means the cleavage could be carried out according to specific spatial and temporal requirements. The biocompatibility of this method makes the DNA strand in combination with riboflavin a promising molecular tool for RNA manipulation. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/27563]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhao, Yongyun,Chen, Gangyi,Yuan, Yi,et al. Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin[J]. SCIENTIFIC REPORTS,2015,5:-. |
| APA | Zhao, Yongyun.,Chen, Gangyi.,Yuan, Yi.,Li, Na.,Dong, Juan.,...&Tang, Zhuo.(2015).Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin.SCIENTIFIC REPORTS,5,-. |
| MLA | Zhao, Yongyun,et al."Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin".SCIENTIFIC REPORTS 5(2015):-. |
入库方式： OAI收割
来源：成都生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。