Metabolic study of Angelica dahurica extracts using a reusable liver microsomal nanobioreactor by liquid chromatography-mass spectrometry
文献类型：期刊论文
| 作者 | Shi, Hai-Li; Jia, Yan-Wei; Peng, Shu-Lin; Liao, Xun; Ding, Li-Sheng; Liu, Yi-Ming |
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2015 |
| 卷号 | 29期号:10页码:1514-1521 |
| 关键词 | RAT PLASMA IN-VITRO MAGNETIC NANOPARTICLES MSN TECHNIQUE IDENTIFICATION COUMARINS FURANOCOUMARINS IMPERATORIN FUROCOUMARINS CONSTITUENTS |
| 产权排序 | 1 |
| 通讯作者 | Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China. |
| 合作状况 | 国际 |
| 英文摘要 | Highly active and recoverable nanobioreactors prepared by immobilizing rat liver microsomes on magnetic nanoparticles (LMMNPs) were utilized in metabolic study of Angelica dahurica extracts. Five metabolites were detected in the incubation solution of the extracts and LMMNPs, which were identified by means of HPLC-MS as trans-imperatorin hydroxylate (M1), cis-imperatorin hydroxylate (M2), imperatorin epoxide (M3), trans-isoimperatorin hydroxylate (M1) and cis-isoimperatorin hydroxylate (speculated M2). Compared with the metabolisms of imperatorin and isoimperatorin, it was found that the five metabolites were all transformed from these two major compounds present in the plant. Since no study on isoimperatorin metabolism by liver microsomal enzyme system has been reported so far, its metabolites (M1 and M3) were isolated by preparative HPLC for structure elucidation by H-1-NMR and MS2 analysis. M3 was identified as isoimperatorin epoxide, which is a new compound as far as its chemical structure is concerned. However, interestingly, M3 was not detected in the metabolism of the whole plant extract. In addition, a study with known chemical inhibitors on individual isozymes of the microsomal enzyme family revealed that CYP1A2 is involved in metabolisms of both isoimperatorin and imperatorin, and CYP3A4 only in that of isoimperatorin. Copyright (c) 2015 John Wiley & Sons, Ltd. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/27568]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Shi, Hai-Li,Jia, Yan-Wei,Peng, Shu-Lin,et al. Metabolic study of Angelica dahurica extracts using a reusable liver microsomal nanobioreactor by liquid chromatography-mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2015,29(10):1514-1521. |
| APA | Shi, Hai-Li,Jia, Yan-Wei,Peng, Shu-Lin,Liao, Xun,Ding, Li-Sheng,&Liu, Yi-Ming.(2015).Metabolic study of Angelica dahurica extracts using a reusable liver microsomal nanobioreactor by liquid chromatography-mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,29(10),1514-1521. |
| MLA | Shi, Hai-Li,et al."Metabolic study of Angelica dahurica extracts using a reusable liver microsomal nanobioreactor by liquid chromatography-mass spectrometry".BIOMEDICAL CHROMATOGRAPHY 29.10(2015):1514-1521. |
入库方式： OAI收割
来源：成都生物研究所
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