The activation of IgM- or isotype-switched IgG- and IgE-BCR exhibits distinct mechanical force sensitivity and threshold
文献类型：期刊论文
| 作者 | Wan, Zhengpeng; Chen, Xiangjun; Chen, Haodong; Ji, Qinghua; Chen, Yingjia; Wang, Jing; Cao, Yiyun; Wang, Fei; Lou, Jizhong; Tang, Zhuo |
| 刊名 | ELIFE
 |
| 出版日期 | 2015 |
| 卷号 | 4页码:- |
| 关键词 | B-CELL RECEPTOR HIGH EPITOPE DENSITY ANTIGEN RECEPTOR IMMUNOLOGICAL SYNAPSES PROTEIN MOLECULE CATCH BONDS IN-VIVO MEMORY TAIL INTEGRIN |
| 产权排序 | 2 |
| 通讯作者 | Liu, WL (reprint author), Tsinghua Univ, Sch Life Sci, Collaborat Innovat Ctr Diag & Treatment Infect Di, MOE Key Lab Prot Sci, Beijing 100084, Peoples R China. |
| 合作状况 | 国内 |
| 英文摘要 | B lymphocytes use B cell receptors (BCRs) to sense the physical features of the antigens. However, the sensitivity and threshold for the activation of BCRs resulting from the stimulation by mechanical forces are unknown. Here, we addressed this question using a double-stranded DNA-based tension gauge tether system serving as a predefined mechanical force gauge ranging from 12 to 56 pN. We observed that IgM-BCR activation is dependent on mechanical forces and exhibits a multi-threshold effect. In contrast, the activation of isotype-switched IgG- or IgE-BCR only requires a low threshold of less than 12 pN, providing an explanation for their rapid activation in response to antigen stimulation. Mechanistically, we found that the cytoplasmic tail of the IgG-BCR heavy chain is both required and sufficient to account for the low mechanical force threshold. These results defined the mechanical force sensitivity and threshold that are required to activate different isotyped BCRs. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Biology |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/27571]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Wan, Zhengpeng,Chen, Xiangjun,Chen, Haodong,et al. The activation of IgM- or isotype-switched IgG- and IgE-BCR exhibits distinct mechanical force sensitivity and threshold[J]. ELIFE,2015,4:-. |
| APA | Wan, Zhengpeng.,Chen, Xiangjun.,Chen, Haodong.,Ji, Qinghua.,Chen, Yingjia.,...&Liu, Wanli.(2015).The activation of IgM- or isotype-switched IgG- and IgE-BCR exhibits distinct mechanical force sensitivity and threshold.ELIFE,4,-. |
| MLA | Wan, Zhengpeng,et al."The activation of IgM- or isotype-switched IgG- and IgE-BCR exhibits distinct mechanical force sensitivity and threshold".ELIFE 4(2015):-. |
入库方式： OAI收割
来源：成都生物研究所
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