Isolation and characterization of Paenibacillus polymyxa LY214, a camptothecin-producing endophytic bacterium from Camptotheca acuminata
文献类型：期刊论文
| 作者 | Pu, Xiang; Chen, Fei; Yang, Yun; Qu, Xixing; Zhang, Guolin; Luo, Yinggang |
| 刊名 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 42期号:8页码:1197-1202 |
| 关键词 | MIQUELIA-DENTATA BEDD. SECONDARY METABOLITES ICACINACEAE FERMENTATION FUNGUS |
| 产权排序 | 1 |
| 通讯作者 | Luo, YG (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ctr Nat Prod Res, Chengdu 610041, Peoples R China. |
| 合作状况 | 国内 |
| 英文摘要 | Camptothecin (CPT) is mainly produced and extracted from Camptotheca acuminata and Nothapodytes foetida for pharmaceutical use, i.e., the starting material for chemical conversion to the clinical CPT-type drugs. As the third largest plant anticancer drug, the heavy demand on CPT from global market leads to many research efforts to identify new sources for CPT production. Herein we report the isolation and characterization of a CPT-producing endophytic bacterium Paenibacillus polymyxa LY214 from Camptotheca acuminata. A 10.7 mu g l(-1) of CPT was presented in the fermentation broth of P. polymyxa LY214. Its CPT production decreased sharply when the strain of the 2nd generation of P. polymyxa LY214 was cultured and fermented. However, the CPT production remained relatively constant from 2.8 mu g l(-1) of the 2nd generation to 0.8 mu g l(-1) of the 8th generation of P. polymyxa LY214 under optimized fermentation conditions. A 15- to 30-fold increase of CPT yield was observed when the optimized fermentation conditions, together with the addition of putative biosynthetic precursors of CPT and adsorbent resin XAD16, were applied to ferment the strains of the 7th and 8th generation of P. polymyxa LY214. Bioinformatics analysis of the relative species of P. polymyxa LY214 indicates its potential to produce CPT, which will be helpful to decipher the mysteries of CPT biosynthesis. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/27575]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Pu, Xiang,Chen, Fei,Yang, Yun,et al. Isolation and characterization of Paenibacillus polymyxa LY214, a camptothecin-producing endophytic bacterium from Camptotheca acuminata[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(8):1197-1202. |
| APA | Pu, Xiang,Chen, Fei,Yang, Yun,Qu, Xixing,Zhang, Guolin,&Luo, Yinggang.(2015).Isolation and characterization of Paenibacillus polymyxa LY214, a camptothecin-producing endophytic bacterium from Camptotheca acuminata.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(8),1197-1202. |
| MLA | Pu, Xiang,et al."Isolation and characterization of Paenibacillus polymyxa LY214, a camptothecin-producing endophytic bacterium from Camptotheca acuminata".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.8(2015):1197-1202. |
入库方式： OAI收割
来源：成都生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。