Untargeted analysis of sesquiterpene pyridine alkaloids from the dried roots of Tripterygium wilfordii using high-performance liquid chromatography/electrospray ionization tandem mass spectrometry
文献类型：期刊论文
| 作者 | Cai, Tian; Luo, Ying-Gang; Zhou, Min; Wang, Dan; Wu, Zhi-Jun; Fang, Dong-Mei; Zhang, Guo-Lin |
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2015 |
| 卷号 | 29期号:10页码:965-972 |
| 关键词 | ANTI-HIV AGENTS PERITASSA-CAMPESTRIS MAYTENUS-EMARGINATA GAS-PHASE HOOK. F IDENTIFICATION HYPOGLAUCUM DERIVATIVES REACTIVITY EXTRACTS |
| 产权排序 | 1 |
| 通讯作者 | Wu, ZJ (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China. |
| 合作状况 | 国内 |
| 英文摘要 | RATIONALE: Sesquiterpene pyridine alkaloids are a large group of highly oxygenated sesquiterpenoids that have attracted attention in the fields of medicine because of their significant biological activities. METHODS: Reference compounds including 14 sesquiterpene pyridine alkaloids and one dihydroagarofuran ester were analyzed by collision-induced dissociation tandem mass spectrometry (CID-MS/MS). A high-performance liquid chromatography/electrospray ionization (HPLC/ESI)-MS/MS method at two collision energies was adopted to investigate the botanical extracts of Tripterygium wilfordii. RESULTS: For 15 reference compounds, in the high mass range, the product ions were formed by the loss of side chains or H2O. In the low mass range, the high-abundance product ions at m/z 206, 204, or 194 were the characteristic ions of the pyridine moiety. The characteristic product ion at m/z 310 was formed through an ion neutral complex intermediate. Fifty-four sesquiterpenoid derivatives, including 50 sesquiterpene pyridine alkaloids, were identified or tentatively characterized in botanical extracts of T. wilfordii based on their elemental constituents, characteristic fragmentation patterns, and the major product ion profiles of the reference compounds ascertained with HPLC/ESI-MS/MS at two collision energies. It seems that isocratic energy was appropriate for the untargeted analysis of compounds with molecular weights exceeding 800 Da, whereas a linear gradient energy vs molecular weight was suitable for those compounds with molecular weights below 800 Da. CONCLUSIONS: The HPLC/ESI-MS/MS method, combining characteristic fragmentation patterns and the profiles of the product ions generated at different collision energies, is an effective technique for characterizing untargeted compounds. Copyright (C) 2015 John Wiley & Sons, Ltd. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry; Spectroscopy |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/27582]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Cai, Tian,Luo, Ying-Gang,Zhou, Min,et al. Untargeted analysis of sesquiterpene pyridine alkaloids from the dried roots of Tripterygium wilfordii using high-performance liquid chromatography/electrospray ionization tandem mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2015,29(10):965-972. |
| APA | Cai, Tian.,Luo, Ying-Gang.,Zhou, Min.,Wang, Dan.,Wu, Zhi-Jun.,...&Zhang, Guo-Lin.(2015).Untargeted analysis of sesquiterpene pyridine alkaloids from the dried roots of Tripterygium wilfordii using high-performance liquid chromatography/electrospray ionization tandem mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,29(10),965-972. |
| MLA | Cai, Tian,et al."Untargeted analysis of sesquiterpene pyridine alkaloids from the dried roots of Tripterygium wilfordii using high-performance liquid chromatography/electrospray ionization tandem mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 29.10(2015):965-972. |
入库方式： OAI收割
来源：成都生物研究所
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