A negative-feedback function of PKC beta in the formation and accumulation of signaling-active B cell receptor microclusters within B cell immunological synapse
文献类型：期刊论文
| 作者 | Liu, Ce; Zhao, XingWang; Xu, LiLing; Yi, JunYang; Shaheen, Samina; Han, Weidong; Wang, Fei; Zheng, Wenjie; Xu, Chenqi; Liu, Wanli |
| 刊名 | JOURNAL OF LEUKOCYTE BIOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 97期号:5页码:887-900 |
| 关键词 | PROTEIN-KINASE-C BRUTONS TYROSINE KINASE ANTIGEN RECEPTOR T-CELLS ACTIVATION EXPRESSION BTK AGAMMAGLOBULINEMIA POLARIZATION INITIATION |
| 产权排序 | 4 |
| 通讯作者 | Liu, WL (reprint author), Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China. |
| 合作状况 | 国内 |
| 英文摘要 | Advanced live cell imaging studies suggested that B cell activation is initiated by the formation of BCR microclusters and subsequent B cell IS upon BCR and antigen recognition. PKC family member PKC beta is highly expressed in B cells and plays an important role in the initiation of B cell activation. Here, we reported an inhibitory function of PKC beta through a negative-feedback manner in B cell activation. Compared with WT (PKC beta-WT) or the constitutively active (PKC beta-DNPS) form of PKC beta, DN PKC beta (PKC beta-DN) unexpectedly enhanced the accumulation of BCR microclusters into the B cell IS, leading to the recruitment of an excessive amount of pSyk, pPLC-gamma 2, and pBLNK signaling molecules into the membrane-proximal BCR signalosome. Enhanced calcium mobilization responses in the decay phase were also observed in B cells expressing PKC beta-DN. Mechanistic studies showed that this negative-feedback function of PKC beta works through the induction of an inhibitory form of pBtk at S180 (pBtk-S180). Indeed, the capability of inducing the formation of an inhibitory pBtk-S180 is in the order of PKC beta-DNPS. PKC beta-WT. PKC beta-DN. Thus, these results improve our comprehensive understanding on the positive and negative function of PKC beta in the fine tune of B cell activation. |
| 学科主题 | Cell Biology; Hematology; Immunology |
| 类目[WOS] | Cell Biology ; Hematology ; Immunology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/27588]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Liu, Ce,Zhao, XingWang,Xu, LiLing,et al. A negative-feedback function of PKC beta in the formation and accumulation of signaling-active B cell receptor microclusters within B cell immunological synapse[J]. JOURNAL OF LEUKOCYTE BIOLOGY,2015,97(5):887-900. |
| APA | Liu, Ce.,Zhao, XingWang.,Xu, LiLing.,Yi, JunYang.,Shaheen, Samina.,...&Liu, Wanli.(2015).A negative-feedback function of PKC beta in the formation and accumulation of signaling-active B cell receptor microclusters within B cell immunological synapse.JOURNAL OF LEUKOCYTE BIOLOGY,97(5),887-900. |
| MLA | Liu, Ce,et al."A negative-feedback function of PKC beta in the formation and accumulation of signaling-active B cell receptor microclusters within B cell immunological synapse".JOURNAL OF LEUKOCYTE BIOLOGY 97.5(2015):887-900. |
入库方式： OAI收割
来源：成都生物研究所
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