An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway
文献类型：期刊论文
| 作者 | Luo, Jing; Li, Caixia; Chen, Jianlin; Wang, Gang; Gao, Rong; Gu, Zhongwei |
| 刊名 | INTERNATIONAL JOURNAL OF NANOMEDICINE
 |
| 出版日期 | 2015 |
| 卷号 | 10页码:1667-1677 |
| 关键词 | MEDIATED ENDOCYTOSIS CATIONIC LIPIDS TRANSFECTION CELLS POLYETHYLENIMINE POLYPLEXES LIPOPLEXES LIPOSOMES POLYMERS VECTORS |
| 产权排序 | 3 |
| 通讯作者 | Wang, G (reprint author), Sichuan Univ, Natl Engn Res Ctr Biomat, 29 Wangjiang Rd, Chengdu 610064, Sichuan, Peoples R China. |
| 合作状况 | 国内 |
| 英文摘要 | Transfection efficiency was the primary goal for in vitro gene delivery mediated by nonviral gene carriers. Here, we report a modified gene transfection method that could greatly increase the efficiency of, and accelerate the process mediated by, 25 kDa branched polyethyleneimine and Lipofectamine (TM) 2000 in a broad range of cell strains, including tumor, normal, primary, and embryonic stem cells. In this method, the combination of transfection procedure with optimized complexation volume had a determinant effect on gene delivery result. The superiorities of the method were found to be related to the change of cellular endocytosis pathway and decrease of particle size. The efficient and simple method established in this study can be widely used for in vitro gene delivery into cultured cells. We think it may also be applicable for many more nonviral gene delivery materials than polyethyleneimine and liposome. |
| 学科主题 | Science & Technology - Other Topics; Pharmacology & Pharmacy |
| 类目[WOS] | Nanoscience & Nanotechnology ; Pharmacology & Pharmacy |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/27606]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Luo, Jing,Li, Caixia,Chen, Jianlin,et al. An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2015,10:1667-1677. |
| APA | Luo, Jing,Li, Caixia,Chen, Jianlin,Wang, Gang,Gao, Rong,&Gu, Zhongwei.(2015).An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway.INTERNATIONAL JOURNAL OF NANOMEDICINE,10,1667-1677. |
| MLA | Luo, Jing,et al."An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway".INTERNATIONAL JOURNAL OF NANOMEDICINE 10(2015):1667-1677. |
入库方式： OAI收割
来源：成都生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。