Diurnal Expression Pattern, Allelic Variation, and Association Analysis Reveal Functional Features of the E1 Gene in Control of Photoperiodic Flowering in Soybean
文献类型：期刊论文
| 作者 | H. Zhai; S. X. Lu; H. Y. Wu; Y. P. Zhang; X. Z. Zhang; J. Y. Yang; Y. Y. Wang; G. Yang; H. M. Qiu; T. T. Cui |
| 刊名 | Plos One
 |
| 出版日期 | 2015 |
| 卷号 | 10期号:8 |
| 通讯作者 | 夏正俊 |
| 中文摘要 | Although four maturity genes, E1 to E4, in soybean have been successfully cloned, their functional mechanisms and the regulatory network of photoperiodic flowering remain to be elucidated. In this study, we investigated how the diurnal expression pattern of the E1 gene is related to photoperiodic length; and to what extent allelic variation in the B3-like domain of the E1 gene is associated with flowering time phenotype. The bimodal expression of the E1 gene peaked first at around 2 hours after dawn in long-day condition. The basal expression level of E1 was enhanced by the long light phase, and decreased by duration of dark. We identified a 5bp (3 SNP and 2-bp deletion) mutation, referred to an e1-b3a, which occurs in the middle of B3 domain of the E1 gene in the early flowering cultivar Yanhuang 3. Subcellular localization analysis showed that the putative truncated e1-b3a protein was predominately distributed in nuclei, indicating the distribution pattern of e1-b3a was similar to that of E1, but not to that of e1-as. Furthermore, genetic analysis demonstrated allelic variations at the E1 locus significantly underlay flowering time in three F-2 populations. Taken together, we can conclude the legume specific E1 gene confers some special features in photoperiodic control of flowering in soybean. Further characterization of the E1 gene will extend our understanding of the soybean flowering pathway in soybean. |
| 源URL | [http://159.226.123.10/handle/131322/6450]  |
| 专题 | 东北地理与农业生态研究所_大豆分子育种学科组 |
| 推荐引用方式 GB/T 7714 | H. Zhai,S. X. Lu,H. Y. Wu,et al. Diurnal Expression Pattern, Allelic Variation, and Association Analysis Reveal Functional Features of the E1 Gene in Control of Photoperiodic Flowering in Soybean[J]. Plos One,2015,10(8). |
| APA | H. Zhai.,S. X. Lu.,H. Y. Wu.,Y. P. Zhang.,X. Z. Zhang.,...&Z. J. Xia.(2015).Diurnal Expression Pattern, Allelic Variation, and Association Analysis Reveal Functional Features of the E1 Gene in Control of Photoperiodic Flowering in Soybean.Plos One,10(8). |
| MLA | H. Zhai,et al."Diurnal Expression Pattern, Allelic Variation, and Association Analysis Reveal Functional Features of the E1 Gene in Control of Photoperiodic Flowering in Soybean".Plos One 10.8(2015). |
入库方式： OAI收割
来源：东北地理与农业生态研究所
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