The Indeterminate Domain Protein ROC1 Regulates Chilling Tolerance via Activation of DREB1B/CBF1 in Rice
文献类型：期刊论文
| 作者 | M. Z. Dou, S. Cheng, B. T. Zhao, Y. H. Xuan and M. L. Shao |
| 刊名 | International Journal of Molecular Sciences
 |
| 出版日期 | 2016 |
| 卷号 | 17期号:3 |
| 中文摘要 | Abiotic stress, including salinity, drought and cold, severely affect diverse aspects of plant development and production. Rice is an important crop that does not acclimate to cold; therefore, it is relatively sensitive to low temperature stress. Dehydration-responsive element-binding protein 1s (DREB1s)/C-repeat binding factors (CBFs) are well known for their function in cold tolerance, but the transcriptional regulation of CBFs remains elusive, especially in rice. Here, we performed a yeast one-hybrid assay using the promoter of CBF1, a cold-induced gene, to isolate transcriptional regulators of CBF1. Among the seven candidates identified, an indeterminate domain (IDD) protein named ROC1 (a regulator of CBF1) was further analyzed. The ROC1 transcript was induced by exogenously-treated auxin, while it was not altered by cold or ABA stimuli. ROC1-GFP was localized at the nucleus, and ROC1 showed trans-activation activity in yeast. The electrophoretic mobility shift assay (EMSA) and ChIP analyses revealed that ROC1 directly bound to the promoter of CBF1. Furthermore, ROC1 mutants exhibited chilling-sensitive symptoms and inhibited cold-mediated induction of CBF1 and CBF3, indicating that ROC1 is a positive regulator of cold stress responses. Taken together, this study identified the CBF1 regulator, and the results are important for rice plant adaptation to chilling stress. |
| 源URL | [http://159.226.123.10/handle/131322/7040]  |
| 专题 | 东北地理与农业生态研究所_湿地生态系统管理学科组 |
| 推荐引用方式 GB/T 7714 | M. Z. Dou, S. Cheng, B. T. Zhao, Y. H. Xuan and M. L. Shao. The Indeterminate Domain Protein ROC1 Regulates Chilling Tolerance via Activation of DREB1B/CBF1 in Rice[J]. International Journal of Molecular Sciences,2016,17(3). |
| APA | M. Z. Dou, S. Cheng, B. T. Zhao, Y. H. Xuan and M. L. Shao.(2016).The Indeterminate Domain Protein ROC1 Regulates Chilling Tolerance via Activation of DREB1B/CBF1 in Rice.International Journal of Molecular Sciences,17(3). |
| MLA | M. Z. Dou, S. Cheng, B. T. Zhao, Y. H. Xuan and M. L. Shao."The Indeterminate Domain Protein ROC1 Regulates Chilling Tolerance via Activation of DREB1B/CBF1 in Rice".International Journal of Molecular Sciences 17.3(2016). |
入库方式： OAI收割
来源：东北地理与农业生态研究所
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