In recent years, an increasing number of environmental contaminants have been found to disrupt endocrine system in wildlife and human. Most research has focused on estrogenic effect; far less attention has been paid to identifying compounds with thyroid disrupting activity in environment. However, in recent years, there has been an increasing concern on the effects of synthetic chemicals on many other nulear receptors disrupting effects. Abundant evidences demonstrating that alkyl esters of p-hydroxybenzoic acid (parabens), phthalates and non-phthalate diester plasticizers have endocrine-disrupting effects. The goal of the current study was to investigate the potential nuclear receptors disruption activities of theses chemicals using two-hybrid system. Molecular docking was performed to simulate the interactions between theses chemicals and the nuclear receptors.