采用乙醇提取、乙酸乙酯萃取、普通硅胶柱色谱和反相硅胶柱色谱分离纯化2',4'-二羟基查尔酮,采用紫外光谱、红外光谱、核磁共振波谱、质谱等技术鉴定结构,应用薄层色谱法、高效液相色谱-二极管阵列检测法、高效液相色谱-蒸发光散射检测法、超高效液相色谱-质谱联用法进行纯度检测。结果从镰形棘豆中分离得到纯度大于99.0%的2',4'-二羟基查尔酮。表明该制备方法简便,制备量大,所制得的2',4'-二羟基查尔酮纯度高,可作为镰形棘豆药材及其复方药的质量控制的对照品。

2',4'-dihydroxychalcone was isolated successively by ethanol extraction, ethyl acetate extraction, silicagel column and reverse phase silica gel column chromatography. The structure of 2',4'-dihydroxychalconewas elucidated by UV, IR, NMR, MS and its purity was detected by TLC, HPLC-PDA, HPLC-ELSD and UPLC-MS. The results showed that the purity of reference substance 2',4'-dihydroxychalcone was higher than99. 0%. The developed method for preparing 2',4'-dihydroxychalcone is simple and provides a high yield, andthe obtained 2',4'-dihydroxychalcone could be used as the chemical reference substance for controlling the qualityof O. falcate and its medicinal preparations.