Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates
文献类型：期刊论文
| 作者 | Wang, Jiancai ; Xu, Ronghua ; Wang, Ruling ; Haque, Mohammad Enamul ; Liu, Aizhong |
| 刊名 | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 80期号:6页码:1214-1222 |
| 中文摘要 | The conversion of acetyl-CoA to malonyl-CoA by acetyl-CoA carboxylase (ACC) is the rate-limiting step in fatty acid biosynthesis. In this study, a gene coding for ACC was isolated and characterized from an oleaginous yeast, Lipomyces starkeyi. Real-time quantitative PCR (qPCR) analysis of L. starkeyi acetyl-CoA carboxylase gene (LsACC1) showed that the expression levels were upregulated with the fast accumulation of lipids. The LsACC1was co-overexpressed with the glycerol 3-phosphate dehydrogenase gene (GPD1), which regulates lipids biosynthesis by supplying another substrates glycerol 3-phosphate for storage lipid assembly, in the non-oleaginous yeast Saccharomyces cerevisiae. Further, the S. cerevisiae acetyl-CoA carboxylase (ScACC1) was transferred with GPD1 and its function was analyzed in comparison with LsACC1. The results showed that overexpressed LsACC1 and GPD1 resulted in a 63% increase in S. cerevisiae. This study gives new data in understanding of the molecular mechanisms underlying the regulation of fatty acids and lipid biosynthesis in yeasts. |
| 公开日期 | 2016-12-09 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/10142]  |
| 专题 | 西双版纳热带植物园_分子遗传和育种研究组 |
| 推荐引用方式 GB/T 7714 | Wang, Jiancai,Xu, Ronghua,Wang, Ruling,et al. Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2016,80(6):1214-1222. |
| APA | Wang, Jiancai,Xu, Ronghua,Wang, Ruling,Haque, Mohammad Enamul,&Liu, Aizhong.(2016).Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,80(6),1214-1222. |
| MLA | Wang, Jiancai,et al."Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 80.6(2016):1214-1222. |
入库方式： OAI收割
来源：西双版纳热带植物园
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