Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler
文献类型:期刊论文
作者 | Gao, Ran1; Lu, Dan-Feng1; Cheng, Jin1; Jiang, Yi2; Jiang, Lan3; Xu, Jian-Dong4; Qi, Zhi-Mei1 |
刊名 | BIOSENSORS & BIOELECTRONICS |
出版日期 | 2016-12-15 |
卷号 | 86期号:DEC页码:321-329 |
ISSN号 | 0956-5663 |
关键词 | Tunable mode coupler Photonic crystal fiber DNA hybridization detection DNA methylation detection Optofluidic biosensor |
英文摘要 | An optical fiber optofluidic biosensor for the detection of DNA hybridization and methylation has been proposed and experimentally demonstrated. An in-line fiber Michelson interferometer was formed in the photonic crystal fiber. A micrhole in the collapsed region, which combined the tunable mode coupler and optofluidic channel, was fabricated by using femtosecond laser micromachining. The mode field diameter of the guided light is changed with the refractive index in the optofluidic channel, which results in the tunable coupling ratio. Label-free detections of the DNA hybridization and methylation have been experimentally demonstrated. The probe single stranded DNA (ssDNA) was bound with the surface of the optofluidic channel through the Poly-L-lysine layer, and the hybridization between a short 22-mer probe ssDNA and a complementary target ssDNA was carried out and detected by interrogating the fringe visibility of the reflection spectrum. Then, the DNA methylation was also detected through the binding between the methylated DNA and the 5-methylcytosine (5-mC) monoclonal antibody. The experiments results demonstrate that the limit of detection of 5 nM is achieved, establishing the tunable mode coupler as a sensitive and versatile biosensor. The sensitive optical fiber optofluidic biosensor possesses high specificity and low temperature cross-sensitivity. (C) 2016 Elsevier B.V. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
类目[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
关键词[WOS] | SURFACE ; INTERFEROMETER ; SYSTEM |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000384853300045 |
源URL | [http://ir.ipe.ac.cn/handle/122111/21612] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Elect, State Key Lab Thansducer Technol, Beijing 100190, Peoples R China 2.Beijing Inst Technol, Sch Optoelect, Beijing 100081, Peoples R China 3.Beijing Inst Technol, Laser Micro Nano Fabricat Lab, Beijing 100081, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Gao, Ran,Lu, Dan-Feng,Cheng, Jin,et al. Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler[J]. BIOSENSORS & BIOELECTRONICS,2016,86(DEC):321-329. |
APA | Gao, Ran.,Lu, Dan-Feng.,Cheng, Jin.,Jiang, Yi.,Jiang, Lan.,...&Qi, Zhi-Mei.(2016).Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler.BIOSENSORS & BIOELECTRONICS,86(DEC),321-329. |
MLA | Gao, Ran,et al."Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler".BIOSENSORS & BIOELECTRONICS 86.DEC(2016):321-329. |
入库方式: OAI收割
来源:过程工程研究所
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