Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes
文献类型：期刊论文
| 作者 | Xu, Shujuan1,2; Li, Qian1; Xiang, Junfeng1; Yang, Qianfan1; Sun, Hongxia1; Guan, Aijiao1; Wang, Lixia1; Liu, Yan1; Yu, Lijia1; Shi, Yunhua1,2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-04-21 |
| 卷号 | 6 |
| 英文摘要 | RNA G-quadruplexes (G4s) play important roles in translational regulation, mRNA processing events and gene expression. Therefore, a fluorescent probe that is capable of efficiently recognizing RNA G-quadruplex structures among other RNA forms is highly desirable. In this study, a water-soluble fluorogenic dye (i.e., Thioflavin T (ThT)) was employed to recognize RNA G-quadruplex structures using UV-Vis absorption spectra, fluorescence spectra and emission lifetime experiments. By stacking on the G-tetrad, the ThT probe exhibited highly specific recognition of RNA G-quadruplex structures with striking fluorescence enhancement compared with other RNA forms. The specific binding demonstrates that ThT is an efficient fluorescence sensor that can distinguish G4 and non-G4 RNA structures. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/36110]  |
| 专题 | 化学研究所_分子动态与稳态结构实验室 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Struct Chem Unstable & Stable Speci, Natl Lab Mol Sci, Ctr Mol Sci,Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Shujuan,Li, Qian,Xiang, Junfeng,et al. Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Xu, Shujuan.,Li, Qian.,Xiang, Junfeng.,Yang, Qianfan.,Sun, Hongxia.,...&Tang, Yalin.(2016).Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes.SCIENTIFIC REPORTS,6. |
| MLA | Xu, Shujuan,et al."Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。