Super-Resolution Optical Subtraction Microscopy Using Optical Scattering Imaging
文献类型：期刊论文
| 作者 | Zhou Qian1,2,3; Yu Jian-Qiang4; Zhao Li-Bo3; Li De-Sheng5; Wu Kui6; Zhu Jian-Hua1,2; Yuan Jing-He3; Fang Xiao-Hong3 |
| 刊名 | ACTA PHYSICO-CHIMICA SINICA
 |
| 出版日期 | 2016-05-15 |
| 卷号 | 32期号:5页码:1123-1128 |
| 关键词 | Super-resolution microscopy Subtraction imaging Non-fluorescence imaging Optical scattering imaging |
| 英文摘要 | The existing form of super-resolution microscopy based on specific fluorescent tagging is unable to obtain super-resolution images of non-fluorescent samples. Hence, we have developed optical subtraction microscopy for obtaining super-resolution imaging in such cases. This method is based on image subtraction between the two optical scattering images from general confocal excitation and doughnut-shaped excitation, respectively. Unlike super-resolution fluorescence microscopy, subtraction microscopy requires no preprocessing of the sample, and the excitation power can be kept low to avoid sample damage. The non-fluorescent imaging of gold nanobeads and polymer nanofibers has been realized to demonstrate the feasibility of super-resolution subtraction microscopy. The lateral resolution decreases to 215 nm (0.33 lambda, 1 lambda = 650 nm) in subtraction imaging, and greater imaging detail of the sample is achieved via optical scattering. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/35760]  |
| 专题 | 化学研究所_分子纳米结构与纳米技术实验室 |
| 作者单位 | 1.Sichuan Univ, Dept Phys, Chengdu 610064, Peoples R China 2.Minist Educ, Key Lab High Energy Dens Phys & Technol, Chengdu 610064, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China 4.North China Univ Technol, Coll Sci, Beijing 100144, Peoples R China 5.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China 6.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou Qian,Yu Jian-Qiang,Zhao Li-Bo,et al. Super-Resolution Optical Subtraction Microscopy Using Optical Scattering Imaging[J]. ACTA PHYSICO-CHIMICA SINICA,2016,32(5):1123-1128. |
| APA | Zhou Qian.,Yu Jian-Qiang.,Zhao Li-Bo.,Li De-Sheng.,Wu Kui.,...&Fang Xiao-Hong.(2016).Super-Resolution Optical Subtraction Microscopy Using Optical Scattering Imaging.ACTA PHYSICO-CHIMICA SINICA,32(5),1123-1128. |
| MLA | Zhou Qian,et al."Super-Resolution Optical Subtraction Microscopy Using Optical Scattering Imaging".ACTA PHYSICO-CHIMICA SINICA 32.5(2016):1123-1128. |
入库方式： OAI收割
来源：化学研究所
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