Computational design of peptide-Au cluster probe for sensitive detection of alpha(IIb)beta(3) integrin
文献类型：期刊论文
| 作者 | Zhao, Lina1; Zhai, Jiao1; Zhang, Xuejie1,2; Gao, Xueyun1; Fang, Xiaohong2; Li, Jingyuan1 |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2016 |
| 卷号 | 8期号:7页码:4203-4208 |
| 英文摘要 | We have designed a novel peptide-Au cluster probe to specifically bind to alpha(IIb)beta(3) integrin. As indicated by molecular dynamics (MD) simulations, the binding mode of the native ligand of alpha(IIb)beta(3) integrin, gamma C peptide, can be realized by the designed probe. More importantly, the peptide-Au probe can provide multiple coating peptides to form additional salt bridges with protein, and the binding stability of the probe is comparable to the native ligand. The designed probe was then successfully synthesized. The specific binding in a cellular environment was validated by colocalization analysis of confocal microscopy. In addition, the binding affinity was confirmed by atomic force microscopy (AFM) based single molecule force spectroscopy. Our results suggest the combination of computational design and experimental verification can be a useful strategy for the development of nanoprobes. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/35858]  |
| 专题 | 化学研究所_分子纳米结构与纳米技术实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lina,Zhai, Jiao,Zhang, Xuejie,et al. Computational design of peptide-Au cluster probe for sensitive detection of alpha(IIb)beta(3) integrin[J]. NANOSCALE,2016,8(7):4203-4208. |
| APA | Zhao, Lina,Zhai, Jiao,Zhang, Xuejie,Gao, Xueyun,Fang, Xiaohong,&Li, Jingyuan.(2016).Computational design of peptide-Au cluster probe for sensitive detection of alpha(IIb)beta(3) integrin.NANOSCALE,8(7),4203-4208. |
| MLA | Zhao, Lina,et al."Computational design of peptide-Au cluster probe for sensitive detection of alpha(IIb)beta(3) integrin".NANOSCALE 8.7(2016):4203-4208. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。