Specific interaction of platinated DNA and proteins by surface plasmon resonance imaging
文献类型：期刊论文
| 作者 | Wang, Xiao1,2; Xu, Jiying1; Liu, Chanjuan1,2; Chen, Yi1,2,3 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:26页码:21900-21906 |
| 英文摘要 | The DNA-targeting platinum complex cisplatin is one of the most successful drugs for clinical treatment of solid tumors, and several new analogues of transplatin have shown cytostatic activity recently. The specific recognition of platinated DNA with cellular proteins is of great interest for better understanding of the pharmaceutical mechanisms. Herein, a surface plasmon resonance imaging (SPRi) method to differentiate the interaction between the protein human high mobility group box 1 (HMGB1) and DNAs, and human nuclear protein positive cofactor 4 (PC4) and DNAs has been developed. Four kinds of DNAs were immobilized on the gold films including platinated-DNA adducts (cisplatin and trans[PtCl2(NH3)(thiazole)] (trans-PtTz) damaged DNAs; referred to as cisPt-DNA1 and transPtTz-DNA2, respectively) and native DNAs (DNA1 and DNA2, as controls). The validation of the method has been proven by the specific recognition of HMGB1 and cisPt-DNA1 first. The results obtained indicated that the PC4 was more likely to bind to platinated DNAs (cisPt-DNA1 and transPtTz-DNA2) than the native DNAs. Temperature-dependent kinetics and thermodynamics revealed that the recognition behavior was not affected by a temperature changefrom 15 degrees C to 42 degrees C. This label-free method provides authentic results, as the controls can be simultaneously determined by a single chip under the same conditions, and this makes it suited for other high throughput analyses of interactions between drug-damaged DNAs and proteins to better understand the activity/inactivity mechanisms of drugs and drug screening/discovery. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/35899]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiao,Xu, Jiying,Liu, Chanjuan,et al. Specific interaction of platinated DNA and proteins by surface plasmon resonance imaging[J]. RSC ADVANCES,2016,6(26):21900-21906. |
| APA | Wang, Xiao,Xu, Jiying,Liu, Chanjuan,&Chen, Yi.(2016).Specific interaction of platinated DNA and proteins by surface plasmon resonance imaging.RSC ADVANCES,6(26),21900-21906. |
| MLA | Wang, Xiao,et al."Specific interaction of platinated DNA and proteins by surface plasmon resonance imaging".RSC ADVANCES 6.26(2016):21900-21906. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。