Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique
文献类型：期刊论文
| 作者 | Jiang, Chao1,2; Yuan, Yuan1; Yang, Guang1; Jin, Yan1; Liu, Libing3; Zhao, Yuyang1; Huang, Luqi1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-10-21 |
| 卷号 | 6 |
| 英文摘要 | Inaccurate labeling of materials used in herbal products may compromise the therapeutic efficacy and may pose a threat to medicinal safety. In this paper, a rapid (within 3 h), sensitive and visual colorimetric method for identifying substitutions in terminal market products was developed using cationic conjugated polymer-based fluorescence resonance energy transfer (CCP-based FRET). Chinese medicinal materials with similar morphology and chemical composition were clearly distinguished by the single-nucleotide polymorphism (SNP) genotyping method. Assays using CCP-based FRET technology showed a high frequency of adulterants in Lu-Rong (52.83%) and Chuan-Bei-Mu (67.8%) decoction pieces, and patented Chinese drugs (71.4%, 5/7) containing Chuan-Bei-Mu ingredients were detected in the terminal herbal market. In comparison with DNA sequencing, this protocol simplifies procedures by eliminating the cumbersome workups and sophisticated instruments, and only a trace amount of DNA is required. The CCP-based method is particularly attractive because it can detect adulterants in admixture samples with high sensitivity. Therefore, the CCP-based detection system shows great potential for routine terminal market checks and drug safety controls. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/35468]  |
| 专题 | 化学研究所_有机固体实验室 |
| 作者单位 | 1.China Acad Chinese Med Sci, State Key Lab Breeding Base Daodi Herbs, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China 2.Beijing Normal Univ, Coll Resources, Beijing Area Major Lab Protect & Utilizat Tradit, Beijing 100875, Peoples R China 3.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Chao,Yuan, Yuan,Yang, Guang,et al. Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Jiang, Chao.,Yuan, Yuan.,Yang, Guang.,Jin, Yan.,Liu, Libing.,...&Huang, Luqi.(2016).Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique.SCIENTIFIC REPORTS,6. |
| MLA | Jiang, Chao,et al."Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：化学研究所
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