Assembly of plasmid DNA with pyrene-amines cationic amphiphiles into nanoparticles and their visible lysosome localization
文献类型：期刊论文
| 作者 | Sheng RL(盛瑞隆)1; AN FEIFEI1; Wang Z(王昭)1; LI MINGRUI1; Cao AM(曹阿民)1 |
| 刊名 | RSC Adv.
 |
| 出版日期 | 2015 |
| 卷号 | 5期号:16页码:12338-12345 |
| 其他题名 | Assembly of plasmid DNA with pyrene-amines cationic amphiphiles into nanoparticles and their visible lysosome localization |
| 通讯作者 | 盛瑞隆 ; 曹阿民 |
| 英文摘要 | In this study, we constructed a visible model for drug/gene dual delivery. Firstly, we prepared a series of pyrene fluorophore bearing cationic amphiphiles (Py-amines), which could be further employed as amphiphilic gene carriers and antitumor polyamine drug models. Then, the Py-amines fluorescent amphiphiles were utilized to bind/load plasmid DNA via electrostatic interactions to form nano-sized particles in aqueous solution. The average size, zeta potential and morphology of the self-assembled Pyamines/ pDNA complexes were found to be largely dependent on the molecular structures of Py-amines amphiphiles. Moreover, the Py-amines and their pDNA complexes showed an evident cell proliferation inhibition capability in H1299 (human lung cancer) cells. Notably, the lysosomal localization of the Pyamines/ pDNA complexes could be directly visualized using fluorescence microscopy in vitro. In summary, this current study could provide new and visible approach to design polyamine-based antitumor drug/plasmid DNA dual delivery systems, which could facilitate a greater understanding of the intracellular trafficking/localization of polyamine-based cationic gene/drug payloads. |
| 学科主题 | 高分子化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1039/c4ra06879c |
| 语种 | 英语 |
| WOS记录号 | WOS:000348985400081 |
| 源URL | [http://ir.sioc.ac.cn/handle/331003/39837]  |
| 专题 | 上海有机化学研究所_高分子材料研究室 |
| 作者单位 | 1.中科院上海有机化学研究所, 有机功能分子合成与组装化学重点实验室 2.国家纳米科学中心 |
| 推荐引用方式 GB/T 7714 | Sheng RL,AN FEIFEI,Wang Z,et al. Assembly of plasmid DNA with pyrene-amines cationic amphiphiles into nanoparticles and their visible lysosome localization[J]. RSC Adv.,2015,5(16):12338-12345. |
| APA | 盛瑞隆,AN FEIFEI,王昭,LI MINGRUI,&曹阿民.(2015).Assembly of plasmid DNA with pyrene-amines cationic amphiphiles into nanoparticles and their visible lysosome localization.RSC Adv.,5(16),12338-12345. |
| MLA | 盛瑞隆,et al."Assembly of plasmid DNA with pyrene-amines cationic amphiphiles into nanoparticles and their visible lysosome localization".RSC Adv. 5.16(2015):12338-12345. |
入库方式： OAI收割
来源：上海有机化学研究所
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