Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase
文献类型：期刊论文
| 作者 | Zhang M(张媚)1; Hou XF(侯现锋)1; Qi LH(漆丽华)1; Yin L(殷乐)1; Li Q(李晴)1; Pan HX(潘海学)1; Chen XY(陈新雅)1; Tang GL(唐功利)1 |
| 刊名 | Chem. Sci.
 |
| 出版日期 | 2015 |
| 卷号 | 6期号:6页码:3440-3447 |
| 其他题名 | 三欣卡辛的生物合成揭示了II型聚酮合酶中新的启示单元及复杂的后修饰 |
| 通讯作者 | 唐功利 |
| 英文摘要 | Trioxacarcins (TXNs) are highly oxygenated, polycyclic aromatic natural products with remarkable biological activity and structural complexity. Evidence from C-13-labelled precursor feeding studies demonstrated that the scaffold was biosynthesized from one unit of L-isoleucine and nine units of malonyl-CoA, which suggested a different starter unit in the biosynthesis. Genetic analysis of the biosynthetic gene cluster revealed 56 genes encoding a type II polyketide synthase (PKS), combined with a large amount of tailoring enzymes. Inactivation of seven post-PKS modification enzymes resulted in the production of a series of new TXN analogues, intermediates, and shunt products, most of which show high anti-cancer activity. Structural elucidation of these new compounds not only helps us to propose the biosynthetic pathway, featuring a type II PKS using a novel starter unit, but also set the stage for further characterization of the enzymatic reactions and combinatorial biosynthesis. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1039/c5sc00116a |
| 语种 | 英语 |
| WOS记录号 | WOS:000354815600022 |
| 源URL | [http://ir.sioc.ac.cn/handle/331003/39507]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 作者单位 | 1.中科院上海有机化学研究所, 生命有机化学国家重点实验室 2.上海生物制造技术协同创新中心 |
| 推荐引用方式 GB/T 7714 | Zhang M,Hou XF,Qi LH,et al. Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase[J]. Chem. Sci.,2015,6(6):3440-3447. |
| APA | 张媚.,侯现锋.,漆丽华.,殷乐.,李晴.,...&唐功利.(2015).Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase.Chem. Sci.,6(6),3440-3447. |
| MLA | 张媚,et al."Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase".Chem. Sci. 6.6(2015):3440-3447. |
入库方式： OAI收割
来源：上海有机化学研究所
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