The SARP Family Regulator Txn9 and Two-Component Response Regulator Txn11 are Key Activators for Trioxacarcin Biosynthesis in Streptomyces bottropensis
文献类型：期刊论文
| 作者 | Yang K(杨魁)1; Qi LH(漆丽华)1; Zhang M(张媚)1; Hou XF(侯现锋)1; Pan HX(潘海学)1; Tang GL(唐功利)1; Wang W(王伟)1; Yuan H(袁华)1 |
| 刊名 | Curr. Microbiol.
 |
| 出版日期 | 2015 |
| 卷号 | 71期号:4页码:458-464 |
| 其他题名 | SARP家族调控蛋白Txn9和双组份调控蛋白Txn11是三欣卡辛生物合成中的正调控因子 |
| 通讯作者 | 王伟 ; 袁华 |
| 英文摘要 | Trioxacarcin A is a polyoxygenated, structurally complex antibiotic produced by Streptomyces spp., which possesses high anti-bacterial, anti-malaria, and anti-tumor activities. The trioxacarcin biosynthetic pathway involves type II polyketide synthases (PKSs) with l-isoleucine as a unique starter unit, as well as many complex post-PKS tailoring enzymes and resistance and regulatory proteins. In this work, two regulatory genes, txn9 coding for a Streptomyces antibiotic regulatory protein family regulator and txn11 for a two-component response regulator, were revealed to be absolutely required for trioxacarcin production by individually inactivating all the six annotated regulatory genes in the txn cluster. Complementation assay suggested that these two activators do not have a regulatory cascade relationship. Moreover, transcriptional analysis showed that they activate 15 of the 28 txn operons, indicating that a complicated regulatory network is involved in the trioxacarcin production. Information gained from this study may be useful for improving the production of the highly potent trioxacarcin A. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1007/s00284-015-0868-9 |
| 语种 | 英语 |
| WOS记录号 | WOS:000359807900005 |
| 源URL | [http://ir.sioc.ac.cn/handle/331003/39518]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 作者单位 | 1.上海应用技术学院 2.中科院上海有机化学研究所, 生命有机化学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang K,Qi LH,Zhang M,et al. The SARP Family Regulator Txn9 and Two-Component Response Regulator Txn11 are Key Activators for Trioxacarcin Biosynthesis in Streptomyces bottropensis[J]. Curr. Microbiol.,2015,71(4):458-464. |
| APA | 杨魁.,漆丽华.,张媚.,侯现锋.,潘海学.,...&袁华.(2015).The SARP Family Regulator Txn9 and Two-Component Response Regulator Txn11 are Key Activators for Trioxacarcin Biosynthesis in Streptomyces bottropensis.Curr. Microbiol.,71(4),458-464. |
| MLA | 杨魁,et al."The SARP Family Regulator Txn9 and Two-Component Response Regulator Txn11 are Key Activators for Trioxacarcin Biosynthesis in Streptomyces bottropensis".Curr. Microbiol. 71.4(2015):458-464. |
入库方式： OAI收割
来源：上海有机化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。