Construction and characterization of bacterial artificial chromosome library of black-handed spider monkey (Ateles geoffroyi)
文献类型：期刊论文
| 作者 | Qian YP1; Jin L1; Su B[*]2 |
| 刊名 | GENOME
 |
| 出版日期 | 2004 |
| 卷号 | 47期号:2页码:239-245 |
| 关键词 | black-handed spider monkeys Ateles geoffroyi BAC library |
| ISSN号 | 0831-2796 |
| 通讯作者 | bing.su@uc.edu |
| 合作状况 | 其它 |
| 英文摘要 | The large-insert genomic DNA library is a critical resource for genome-wide genetic dissection of target species. We constructed a high-redundancy bacterial artificial chromosome (BAC) library of a New World monkey species, the black-handed spider monkey (Ateles geoffroyi). A total of 193 152 BAC clones were generated in this library. The average insert size of the BAC clones was estimated to be 184.6 kb with the small inserts (50-100 kb) accounting for less than 3% and the non-recombinant clones only 1.2%. Assuming a similar genome size with humans, the spider monkey BAC library has about 11x genome coverage. In addition, by end sequencing of randomly selected BAC clones, we generated 367 sequence tags for the library. When blasted against human genome, they showed a good correlation between the number of hit clones and the size of the chromosomes, an indication of unbiased chromosomal distribution of the library. This black-handed spider monkey BAC library would serve as a valuable resource in comparative genomic study and large-scale genome sequencing of nonhuman primates. |
| 收录类别 | SCI |
| 原文出处 | 200447239.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2117]  |
| 专题 | 昆明动物研究所_比较基因组学 昆明动物研究所_细胞与分子进化重点实验室 |
| 作者单位 | 1.Center for Genome Information, Department of Environmental Health, University of Cincinnati, 3223 Eden Ave., Cincinnati, OH 45267, U.S.A 2.Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, China |
| 推荐引用方式 GB/T 7714 | Qian YP,Jin L,Su B[*]. Construction and characterization of bacterial artificial chromosome library of black-handed spider monkey (Ateles geoffroyi)[J]. GENOME,2004,47(2):239-245. |
| APA | Qian YP,Jin L,&Su B[*].(2004).Construction and characterization of bacterial artificial chromosome library of black-handed spider monkey (Ateles geoffroyi).GENOME,47(2),239-245. |
| MLA | Qian YP,et al."Construction and characterization of bacterial artificial chromosome library of black-handed spider monkey (Ateles geoffroyi)".GENOME 47.2(2004):239-245. |
入库方式： OAI收割
来源：昆明动物研究所
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