Proteomic Analysis of Proteins Involved in Spermiogenesis in Mouse
文献类型：期刊论文
| 作者 | Guo XJ1; Shen J1; Xia ZR1; Zhang R2; Zhang P1; Zhao C1; Xing J1; Chen L1; Chen W1; Lin M1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2010 |
| 卷号 | 9期号:3页码:1246-1256 |
| 关键词 | spermiogenesis proteome acrosome biogenesis translational regulation |
| ISSN号 | 1535-3893 |
| 通讯作者 | sub@mail.kiz.ac.cn ; zhouzm@njmu.edu.cn |
| 中文摘要 | Spermiogenesis is a unique process in mammals during which haploid round spermatids mature into spermatozoa in the testis. Its successful completion is necessary for fertilization and its malfunction is an important cause of male infertility. Here, we report the high-confidence identification of 2116 proteins in mouse haploid germ cells undergoing spermiogenesis: 299 of these were testis-specific and 155 were novel. Analysis of these proteins showed many proteins possibly functioning in unique processes of spermiogenesis. Of the 84 proteins annotated to be involved in vesicle-related events, VAMP4 was shown to be important for acrosome biogenesis by in vivo knockdown experiments. Knockdown of VAMP4 caused defects of acrosomal vesicle fusion and significantly increased head abnormalities in spermatids from testis and sperm from the cauda epididymis. Analysis of chromosomal distribution of the haploid genes showed underrepresentation on the X chromosome and overrepresentation on chromosome 11, which were due to meiotic sex chromosome inactivation and expansion of testis-expressed gene families, respectively. Comparison with transcriptional data showed translational regulation during spermiogenesis. This characterization of proteins involved in spermiogenesis provides an inventory of proteins useful for understanding the mechanisms of male infertility and may provide candidates for drug targets for male contraception and male infertility. |
| 学科主题 | Biochemical Research Methods |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the National Natural Science Foundation of China (30425011 and 305303800), the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201) and the Innovation Project of Chinese Academy of Sciences (KSCX2-YW-R-090). |
| 原文出处 | 2010931246.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2207]  |
| 专题 | 昆明动物研究所_比较基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Kunming 650223, Yunnan, China |
| 推荐引用方式 GB/T 7714 | Guo XJ,Shen J,Xia ZR,et al. Proteomic Analysis of Proteins Involved in Spermiogenesis in Mouse[J]. JOURNAL OF PROTEOME RESEARCH,2010,9(3):1246-1256. |
| APA | Guo XJ.,Shen J.,Xia ZR.,Zhang R.,Zhang P.,...&Sha JH.(2010).Proteomic Analysis of Proteins Involved in Spermiogenesis in Mouse.JOURNAL OF PROTEOME RESEARCH,9(3),1246-1256. |
| MLA | Guo XJ,et al."Proteomic Analysis of Proteins Involved in Spermiogenesis in Mouse".JOURNAL OF PROTEOME RESEARCH 9.3(2010):1246-1256. |
入库方式： OAI收割
来源：昆明动物研究所
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