MCPH1/BRIT1 represses transcription of the human telomerase reverse transcriptase gene
文献类型：期刊论文
| 作者 | Shi L1,2; Li M1,2; Su B[*]1 |
| 刊名 | GENE
 |
| 出版日期 | 2012 |
| 卷号 | 495期号:1页码:1-9 |
| 关键词 | Microcephalin Transcriptional repressor Telomerase activity |
| 通讯作者 | sub@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | MCPH1, a repressor of human telomerase reverse transcriptase (hTERT) function, is implicated in cellular immortalization. But little is known about how MCPH1 represses telomerase activity. In this study, to determine the mechanism by which MCPH1 regulates hTERT gene expression, we examined the role of MCPH1 in regulating the hTERT promoter in vitro. Co-transfection of the hTERT promoter with MCPH1 in Hela cells could inhibit the hTERT promoter activity. The EMSA assay demonstrated that MCPH1 could bind to the proximal hTERT promoter. Overexpression of MCPH1 could repress telomerase activity, and the repression was abolished by knocking down the MCPH1 expression using siRNA in U2OS cells. We propose that MCPH1 functions as a transcriptional repressor of hTERT in vitro. Since the activation of telomerase, widely observed in human tumor cell lines, is a critical step in tumorigenesis, our findings provide new insights into delineating the tumor-suppressing function of MCPH1 through its down-regulation of hTERT/telomerase expression |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the National 973 project of China (grant number, 2011CBA00401), the National Natural Science Foundation of China (grant numbers, 30871343 and 31071101), and the Natural Science Foundation of Yunnan Province of China (grant numbers, 2007C100M and 2009CD107). |
| 语种 | 英语 |
| WOS记录号 | WOS:000301163600001 |
| 公开日期 | 2012-04-05 |
| 源URL | [http://159.226.149.42:8088/handle/152453/6901]  |
| 专题 | 昆明动物研究所_比较基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_转化基因组 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, PR China 2.Graduate School of Chinese Academy of Sciences, Beijing, PR China |
| 推荐引用方式 GB/T 7714 | Shi L,Li M,Su B[*]. MCPH1/BRIT1 represses transcription of the human telomerase reverse transcriptase gene[J]. GENE,2012,495(1):1-9. |
| APA | Shi L,Li M,&Su B[*].(2012).MCPH1/BRIT1 represses transcription of the human telomerase reverse transcriptase gene.GENE,495(1),1-9. |
| MLA | Shi L,et al."MCPH1/BRIT1 represses transcription of the human telomerase reverse transcriptase gene".GENE 495.1(2012):1-9. |
入库方式： OAI收割
来源：昆明动物研究所
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