Peak identification for ChIP-seq data with no controls
文献类型：期刊论文
| 作者 | Zhang YF; Su B[*] |
| 刊名 | Zoological Research
 |
| 出版日期 | 2012 |
| 卷号 | 33期号:E5-6页码:E121−E128 |
| 关键词 | ChIP-seq Bayesian Peak calling Gene regulation |
| 通讯作者 | sub@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is increasingly being used for genome-wide profiling of transcriptional regulation, as this technique enables dissection of the gene regulatory networks. With input as control, a variety of statistical methods have been proposed for identifying the enriched regions in the genome, i.e., the transcriptional factor binding sites and chromatin modifications. However, when there are no controls, whether peak calling is still reliable awaits systematic evaluations. To address this question, we used a Bayesian framework approach to show the effectiveness of peak calling without controls (PCWC). Using several different types of ChIP-seq data, we demonstrated the relatively high accuracy of PCWC with less than a 5% false discovery rate (FDR). Compared with previously published methods, e.g., the model-based analysis of ChIP-seq (MACS), PCWC is reliable with lower FDR. Furthermore, to interpret the biological significance of the called peaks, in combination with microarray gene expression data, gene ontology annotation and subsequent motif discovery, our results indicate PCWC possesses a high efficiency. Additionally, using in silico data, only a small number of peaks were identified, suggesting the significantly low FDR for PCWC. |
| 收录类别 | 其他 |
| 资助信息 | This study was supported by the National 973 project of China (2011CBA01101) and the National Natural Science Foundation of China (30871343 and 31130051) |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7642]  |
| 专题 | 昆明动物研究所_比较基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Zhang YF,Su B[*]. Peak identification for ChIP-seq data with no controls[J]. Zoological Research,2012,33(E5-6):E121−E128. |
| APA | Zhang YF,&Su B[*].(2012).Peak identification for ChIP-seq data with no controls.Zoological Research,33(E5-6),E121−E128. |
| MLA | Zhang YF,et al."Peak identification for ChIP-seq data with no controls".Zoological Research 33.E5-6(2012):E121−E128. |
入库方式： OAI收割
来源：昆明动物研究所
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