Functional divergence of the rapidly evolving miR-513 subfamily in primates
文献类型：期刊论文
| 作者 | Sun ZH1,2; Zhang YF1,2; Zhang R1,2; Qi XB1,2; Su B[*]1,2 |
| 刊名 | BMC EVOLUTIONARY BIOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 13期号:X页码:e255 |
| 关键词 | miR-513 Primate-specific Rapid evolution Testis DR1 |
| 通讯作者 | sub@mail.kiz.ac.cn |
| 英文摘要 | Background: The miR-513 subfamily belongs to an X-linked primate-specific miR506-514 cluster. Across primate species, there have been several duplication events and different species each possess a variety of miR-513 copies, indicating it underwent rapid evolution. Evidence suggests that this subfamily is preferentially expressed in the testis, but otherwise, to date, the evolutionary history and functional significance of this miRNA subfamily has remained largely unexplored. Results: We analyzed the evolutionary pattern of gene duplications and their functional consequence for the miR-513 subfamily in primates. Sequence comparisons showed that the duplicated copies of miR-513 were derived from transposable element (MER91C). Moreover, duplication events of the miR-513 subfamily seem to have occurred independently in Platyrrhini (New World monkeys) and Catarrhini (Old World monkeys, apes and humans) after they diverged. Different copies of the miR-513 subfamily (miR-513a/b/c) have different seed sequences, due to after-duplication sequence divergences, which eventually led to functional divergences. The results of functional assays indicated that miR-513b could inhibit the expression of its target gene, the down-regulator of transcription 1 (DR1) at both the mRNA and protein levels. In the developing testis of rhesus macaques, we observed a temporal coupling of expression levels between miR-513b and DR1, suggesting that miR-513b could affect male sexual maturation by negatively regulating the development-stage related functioning of DR1. Conclusions: The miR-513 subfamily underwent multiple independent gene duplications among five different lineages of primates. The after-duplication sequence divergences among the different copies of miR-513 led to functional divergence of these copies in primates. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the National 973 project of China (2012CBA01304) and the National Natural Science Foundation of China (31130051). |
| 语种 | 英语 |
| WOS记录号 | WOS:000328461500002 |
| 公开日期 | 2014-01-10 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7769]  |
| 专题 | 昆明动物研究所_比较基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Yunnan Key Laboratory of Primate Biomedical Research, Kunming 650217, China 2.Kunming Institute of Zoology, State Key Laboratory of Genetic Resources and Evolution, Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Sun ZH,Zhang YF,Zhang R,et al. Functional divergence of the rapidly evolving miR-513 subfamily in primates[J]. BMC EVOLUTIONARY BIOLOGY,2013,13(X):e255. |
| APA | Sun ZH,Zhang YF,Zhang R,Qi XB,&Su B[*].(2013).Functional divergence of the rapidly evolving miR-513 subfamily in primates.BMC EVOLUTIONARY BIOLOGY,13(X),e255. |
| MLA | Sun ZH,et al."Functional divergence of the rapidly evolving miR-513 subfamily in primates".BMC EVOLUTIONARY BIOLOGY 13.X(2013):e255. |
入库方式： OAI收割
来源：昆明动物研究所
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