Age-related gene expression change of GABAergic system in visual cortex of rhesus macaque.
文献类型：期刊论文
| 作者 | Liao CH1,2; Han Q1,2; Ma YY3,4,5; Su B[*]6 |
| 刊名 | GENE
 |
| 出版日期 | 2016 |
| 卷号 | 590期号:2页码:227-233 |
| 关键词 | Aging Compensatory GABA Gene expression Vision |
| 通讯作者 | sub@mail.kiz.ac.cn |
| 英文摘要 | Degradation of visual function is a common phenomenon during aging and likely mediated by change in the impaired central visual pathway. Treatment with GABA or its agonist could recover the ability of visual neurons in the primary visual cortex of senescent macaques. However, little is known about how GABAergic system change is related to the aged degradation of visual function in nonhuman primate. With the use of quantitative PCR method, we measured the expression change of 24 GABA related genes in the primary visual cortex (Brodmann's 17) of different age groups. In this study, both of mRNA and protein of glutamic acid decarboxylase (GAD65) were measured by real-time RT-PCR and Western blot, respectively. Results revealed that the level of GAD65 message was not significantly altered, but the proteins were significantly decreased in the aged monkey. As GAD65 plays an important role in GABA synthesis, the down-regulation of GAD65 protein was likely the key factor leading to the observed GABA reduction in the primary visual cortex of the aged macaques. In addition, 7 of 14 GABA receptor genes were up-regulated and one GABA receptor gene was significantly reduced during aging process even after Banjamini correction for multiple comparisons (P<0.05). These results suggested that the dysregulation of GAD65 protein might contribute to some age-related neural visual dysfunctions and most of GABA receptor genes induce a clear indication of compensatory effect for the reduced GABA release in the healthy aged monkey cortex. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the National 973 project of China (2011CBA00401 and 2012CBA01300), the National Natural Sci- ence Foundation of China (31130051, 31301028 and 31321002) and the Natural Science Foundation of Yunnan Province (2007C100M and 2009CD107). This study was also supported by funding from the West Light doctoral program. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9917]  |
| 专题 | 昆明动物研究所_比较基因组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_认知障碍病理学 |
| 作者单位 | 1.Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, 570228, China 2.Laboratory of Tropical Veterinary Medicine and Vector Biology, College of Agriculture, Hainan University, Haikou, 570228, China 3.Laboratory of the Primate Model for Brain Diseases and Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China 4.State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China 5.Yunnan Key Laboratory of Primate Biomedical Research, Kunming, Yunnan, China 6.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Liao CH,Han Q,Ma YY,et al. Age-related gene expression change of GABAergic system in visual cortex of rhesus macaque.[J]. GENE,2016,590(2):227-233. |
| APA | Liao CH,Han Q,Ma YY,&Su B[*].(2016).Age-related gene expression change of GABAergic system in visual cortex of rhesus macaque..GENE,590(2),227-233. |
| MLA | Liao CH,et al."Age-related gene expression change of GABAergic system in visual cortex of rhesus macaque.".GENE 590.2(2016):227-233. |
入库方式： OAI收割
来源：昆明动物研究所
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