Construction of a BAC library for Chinese amphioxus Branchiostoma belcheri and identification of clones containing Amphi-pax genes
文献类型：期刊论文
| 作者 | Wang W1,2; Xu HL2; Lin LP1; Su B2; Wang YQ[*]1 |
| 刊名 | GENES & GENETIC SYSTEMS
 |
| 出版日期 | 2005 |
| 卷号 | 80期号:3页码:233-236 |
| 关键词 | amphioxus Amphi-Pax genes bacterial artifcial chromosome (BAC) library Branchiostoma belcheri |
| 通讯作者 | wangyq@xmu.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | Amphioxus is a crucial organism for the study of vertebrate evolution. Although a genomic BAC library of Branchiostoma floridae has been constructed, we report here another BAC library construction of its distant relative species Branchiostoma belcheri. The amphioxus BAC library established in present study consists of 45,312 clones arrayed in one hundred and eighteen 384-well plates. The average insert fragment size was 120 kb estimated by Pulsed Field Gel Electrophoresis (PFGE) analysis of 318 randomly selected clones. The representation of the library is about 12 equivalent to the genome, allowing a 99.9995% probability of recovering any specific sequence of interest. We further screened the library with 4 single copied Amphi-Pax genes and identified total of 26 positive clones with average of 6.5 clones for each gene. The result indicates this library is well suited for many applications and should also serve as a useful complemental resource for the scientific community. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10604]  |
| 专题 | 昆明动物研究所_比较基因组学 昆明动物研究所_细胞与分子进化重点实验室 |
| 作者单位 | 1.Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University 2.Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | Wang W,Xu HL,Lin LP,et al. Construction of a BAC library for Chinese amphioxus Branchiostoma belcheri and identification of clones containing Amphi-pax genes[J]. GENES & GENETIC SYSTEMS,2005,80(3):233-236. |
| APA | Wang W,Xu HL,Lin LP,Su B,&Wang YQ[*].(2005).Construction of a BAC library for Chinese amphioxus Branchiostoma belcheri and identification of clones containing Amphi-pax genes.GENES & GENETIC SYSTEMS,80(3),233-236. |
| MLA | Wang W,et al."Construction of a BAC library for Chinese amphioxus Branchiostoma belcheri and identification of clones containing Amphi-pax genes".GENES & GENETIC SYSTEMS 80.3(2005):233-236. |
入库方式： OAI收割
来源：昆明动物研究所
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