猕猴精浆纤溶酶原激活因子的来源及在精子获能中的作用
文献类型：期刊论文
| 作者 | 郑萍1; 邹如金1; 刘以训[*]2 |
| 刊名 | 生理学报
 |
| 出版日期 | 2001 |
| 卷号 | 53期号:1页码:45-50 |
| 关键词 | 猕猴 纤溶酶原激活因子 精子获能 |
| ISSN号 | 0371-0874 |
| 其他题名 | Source of plasminogen activator in rhesus monkey semen and its possible role in sperm capacitation |
| 通讯作者 | liuyx@panda.ioz.ac.cn |
| 中文摘要 | 猕猴附睾、前列腺和精囊均表达tPA、uPA和PAI-1 mRNAs。加入uPA能维持精子的活力,使精子产生超激活运动,诱导顶体反应的发生,并使精子获得激活卵子的能力。这说明猕猴精浆PA除来源于睾丸外,可能主要来源于附睾及附性腺;在体外,uPA,而不是tPA,可能诱导精子获能。 |
| 英文摘要 | Previous studies have demonstrated that plasminogen activator (PA) activity is significantly high in semen of infertile men, which is also high in semen when azoospermia or oligozoospermia is induced by injection of testosterone enanthate (TE) into healthy adult men or rhesus monkeys. To further clarify the source and possible role of PA in semen, the present study was undertaken to examine: (1) whether the mRNAs for tissue type PA (tPA), urokinase type PA (uPA), and PA inhibitor-1 (PAI-1) are expressed in epididymis, seminal vesicle and prostate gland of rhesus monkeys; and (2) whether PA has some effect on in vitro sperm capacitation as judged by the potential of sperm motility, acrosome reaction (AR) and in vitro fertilization. Our results showed that (1) mRNAs for PA and PAI-1 were expressed in epididymis, seminal vesicle and prostate gland, and (2) uPA, but not tPA, improved sperm mobility, induced AR and enhanced sperm capacity to fertilize mature eggs. Thus, it is concluded that PA activity in semen comes not only from testis and epididymis, but also from seminal vesicle and prostate gland; and that uPA, but not tPA, may play a role in sperm capacitation. |
| 收录类别 | 其他 |
| 资助信息 | This research was supported by the National Natural Science Foundation of China (No 39810 36 8) ;the Ministry of Science and Technology of China; |
| 语种 | 中文 |
| 公开日期 | 2010-08-23 |
| 源URL | [http://159.226.149.42:8088/handle/152453/1884]  |
| 专题 | 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_其他 |
| 作者单位 | 1.中国科学院昆明动物研究所生殖生物学实验室!昆明650223 2.中国科学院动物研究所生殖生物学国家重点实验室!北京100080； |
| 推荐引用方式 GB/T 7714 | 郑萍,邹如金,刘以训[*]. 猕猴精浆纤溶酶原激活因子的来源及在精子获能中的作用[J]. 生理学报,2001,53(1):45-50. |
| APA | 郑萍,邹如金,&刘以训[*].(2001).猕猴精浆纤溶酶原激活因子的来源及在精子获能中的作用.生理学报,53(1),45-50. |
| MLA | 郑萍,et al."猕猴精浆纤溶酶原激活因子的来源及在精子获能中的作用".生理学报 53.1(2001):45-50. |
入库方式： OAI收割
来源：昆明动物研究所
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