Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development
文献类型：期刊论文
| 作者 | Zheng P1,3; Wang H1,3; Bavister BD2,3; Ji W[*]1,3 |
| 刊名 | HUMAN REPRODUCTION
 |
| 出版日期 | 2001 |
| 卷号 | 16期号:2页码:300-305 |
| 关键词 | blastocysts in-vitro maturation/primate oocytes protein-free medium |
| ISSN号 | 0268-1161 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | This study compared success of in-vitro maturation of rhesus monkey oocytes in protein-free versus serum-containing culture systems, assessed by embryo development subsequent to IVF. Four media were tested: (i) modified Connaught Medical Research Laboratories medium (mCMRL-1066); (ii) hamster embryo culture medium-10 (HECM-10); (iii) control: mCMRL-1066 + 20% bovine calf serum (BCS); (iv) HECM-10 + 20% BCS. Immature oocytes from FSH-stimulated rhesus monkeys were allocated among the media containing ovine FSH (5 microg/ml) and LH (10 microg/ml) and cultured for 36-40 h. Metaphase II ova were inseminated and putative zygotes were cultured in mCMRL-1066 + 20% BCS until development arrested. Ova matured in all four media had similar (P> 0.05) potential to initiate (66, 67, 82 and 69% respectively) and complete meiotic maturation (60, 50, 76 and 57% respectively). Inseminated ova in all groups had similar potential to be fertilized (86, 83, 84 and 90% respectively), cleave (71, 83, 76 and 90% respectively) and develop to the blastocyst stage (19, 17, 16 and 30% respectively). These results indicate for the first time that primate oocytes can be successfully matured in protein-free medium, with subsequent blastocyst development, comparable to responses in complex medium with serum. This finding will facilitate studies on mechanisms regulating primate oocyte maturation. |
| 收录类别 | SCI |
| 原文出处 | 200116300.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2397]  |
| 专题 | 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_生殖与发育生物学 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Zool, Dept Reprod Biol, Kunming 650223, Yunnan, Peoples R China 2.Univ Wisconsin, Dept Anim Hlth & Biomed Sci, Madison, WI 53706 USA 3.Chinese Acad Sci, Kunming Inst Zool, China US Primate Biol Lab, Kunming 650223, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng P,Wang H,Bavister BD,et al. Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development[J]. HUMAN REPRODUCTION,2001,16(2):300-305. |
| APA | Zheng P,Wang H,Bavister BD,&Ji W[*].(2001).Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development.HUMAN REPRODUCTION,16(2),300-305. |
| MLA | Zheng P,et al."Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development".HUMAN REPRODUCTION 16.2(2001):300-305. |
入库方式： OAI收割
来源：昆明动物研究所
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