Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys
文献类型：期刊论文
| 作者 | Zheng P1,3; Bavister BD2,3,4; Ji WZ[*]1,3 |
| 刊名 | MOLECULAR REPRODUCTION AND DEVELOPMENT
 |
| 出版日期 | 2001 |
| 卷号 | 58期号:3页码:348-355 |
| 关键词 | culture media glucose lactate pyr- uvate in vitro fertilization embryo development cumulus expansion |
| ISSN号 | 1040-452X |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 英文摘要 | The energy substrates lactate, pyruvate, and glucose were evaluated for supporting in vitro cytoplasmic maturation of rhesus monkey oocytes. A total of 321 cumulus-oocyte complexes (COCs) aspirated from > or = 1000 microm diameter follicles of unstimulated adult monkeys were matured in one of six media with various individual or combinations of energy substrates: (1) mCMRL-1066 (control); (2) HECM-10 (containing 4.5 mM lactate); (3) HECM-10+0.2 mM pyruvate; (4) HECM-10 + 5.0 mM glucose; (5) HECM-10+ 0.2 mM pyruvate + 5.0 mM glucose; and (6) HECM-10 minus lactate + 5.0 mM glucose. All media contained gonadotropins, oestradiol, and progesterone. Following maturation, all mature oocytes were subjected to the same in vitro fertilization and embryo culture procedures. Oocytes matured in control medium or in treatment groups 4 and 6 had the best morulae+ blastocysts developmental responses (35, 36, and 32%, respectively, P < 0.05). HECM-10 + 0.2 mM pyruvate + 5.0 mM glucose for COC maturation supported intermediate embryonic development (16% morulae + blastocysts). The lowest (P < 0.05) morula + blastocyst developmental responses were obtained after maturation of COCs in HECM-t10 and HECM-10 + 0.2 mM pyruvate (4 and 6%, respectively). The COCs matured in glucose-containing medium showed greater levels of cumulus expansion than those in glucose-free medium. These results indicate that (a) glucose is both necessary and sufficient as the energy substrate for supporting optimal cytoplasmic maturation in vitro of oocytes from unstimulated rhesus monkeys; (b) pyruvate suppresses the stimulatory effect of glucose on oocyte maturation; (c) glucose is involved in cumulus expansion; (d) cumulus expansion is not a reliable indicator of primate oocyte competence. |
| 收录类别 | 其他 |
| 资助信息 | Talent Fund of Yunnan Province, China; Grant number: 960364; Grant sponsor: Major State Research Development Program; Grant number: G2000016108; Grant sponsor: National Institutes of Health; Grant numbers: RR13439, RO3-TW00840, HD22023. |
| 原文出处 | 200158348.pdf |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2399]  |
| 专题 | 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_生殖与发育生物学 |
| 作者单位 | 1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2.Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, Wisconsin 3.Chinese-US Primate Biology Laboratory, Kunming, China, and Madison, Wisconsin 4.Present address: Audubon Institute, Center for Research of Endangered Species, 14001 River Road, New Orleans, LA 70131. |
| 推荐引用方式 GB/T 7714 | Zheng P,Bavister BD,Ji WZ[*]. Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys[J]. MOLECULAR REPRODUCTION AND DEVELOPMENT,2001,58(3):348-355. |
| APA | Zheng P,Bavister BD,&Ji WZ[*].(2001).Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys.MOLECULAR REPRODUCTION AND DEVELOPMENT,58(3),348-355. |
| MLA | Zheng P,et al."Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys".MOLECULAR REPRODUCTION AND DEVELOPMENT 58.3(2001):348-355. |
入库方式： OAI收割
来源：昆明动物研究所
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