年龄和FSH在肉牛活体取卵过程中对卵泡和卵母细胞发育的影响
文献类型：期刊论文
| 作者 | 苏雷; 和协超; 马峻; 季维智[*] |
| 刊名 | 动物学研究
 |
| 出版日期 | 2002 |
| 卷号 | 23期号:5页码:372-378 |
| 关键词 | 肉牛 活体取卵 卵泡 卵母细胞 FSH |
| ISSN号 | 0254-5853 |
| 其他题名 | Effect of Age and Hormonal Stimulation on Development of Beef Follicles and Oocytes During Ultrasound- Guided Transvaginal Follicular Aspiration |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 中文摘要 | 用B超活体取卵技术(TVFA)对莫累灰和婆罗门杂交肉牛取卵,探讨年龄和FSH诱导对青年牛、经产牛及老年牛卵泡和卵母细胞发育的影响.结果显示:青年牛(12月龄)和经产牛(7～8岁)平均卵泡数(≥2 mm)及＞10 mm卵泡数明显高于老年牛(≥14岁)(P＜0.01).青年牛和经产牛平均卵母细胞数及平均COC数高于老年牛(P＜0.05).1和2级卵母细胞比例青年牛高于或等于经产牛高于老年牛,3和4级卵母细胞比例老年牛高于经产牛高于或等于青年牛(P＜O.05).青年牛和经产牛2级卵母细胞占优势,而老年牛3级卵母细胞居多.不同剂量外源性FSH诱导后,经产牛和老年牛平均卵泡数在各剂量组之间没有显著差异(P＞0.05);但≥6 mm卵泡数及2级卵母细胞比例明显增加,而2～5 mm卵泡数相应减少及裸卵和老化卵比例降低(P＜0.05).在10次取卵过程中,青年牛和经产牛发情当天平均卵母细胞数均较以后各次的数量低(P＜0.05).研究表明:利用TVFA可从肉牛获取卵母细胞;卵泡及卵母细胞发育受年龄的影响,这可能与生理状况有关;而适量FSH诱导可以促进牛卵泡发育,提高卵母细胞质量. |
| 英文摘要 | The effect of age and exogenous hormonal stimulation on development of beef (Murray Grey×Brahman) follicles and oocytes were investigated during ultrasound-guided transvaginal follicular aspiration (TVFA).Results showed:mean number of follicles (≥2 mm) and number of follicles ＞10 mm in diameter were higher in heifers and pluriparous than those in older beefs (P＜0.01);mean number of oocytes and COC were higher in the heifers and pluriparous than those in the older beefs (P＜0.05).Among heifers,pluriparous and older beefs,percentage of Ⅰ and Ⅱ grade oocytes were successively decrease,and percentage of Ⅲ and Ⅳ grade ones were opposite (P＜0.05).Ⅱ grade oocytes were more than others in the heifers and pluriparous,and Ⅲ grade oocytes were more than others in the older beefs.After pluriparous or older beefs stimulated by FSH,mean number of follicles was not different among different groups (P＞0.05),but number of follicles ＞6 mm in diameter was increased and 2-5 mm in diameter was accordingly decreased (P＜0.05),percentage of Ⅱ grade oocytes was increased,and percentage of denuded and degenerated oocytes was deceased.Mean number of follicles,oocytes and COC in the heifers and pluriparous were lower at the aspiration of estrus day than that at the other aspirations.In conclusion,oocytes can be obtained from live beefs,age can affect development of beef follicles and oocytes that could base on different physiological state,and appropriate exogenous hormonal stimulation can improve follicular development. |
| 收录类别 | 其他 |
| 资助信息 | 云南省自然科学基金资助项目 (96C006Z);云南省省院省校合作项目 (YK98004) |
| 原文出处 | 2002235372.pdf |
| 语种 | 中文 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4425]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_哺乳动物胚胎发育 |
| 作者单位 | 中国科学院昆明动物研究所,昆明650223 |
| 推荐引用方式 GB/T 7714 | 苏雷,和协超,马峻,等. 年龄和FSH在肉牛活体取卵过程中对卵泡和卵母细胞发育的影响[J]. 动物学研究,2002,23(5):372-378. |
| APA | 苏雷,和协超,马峻,&季维智[*].(2002).年龄和FSH在肉牛活体取卵过程中对卵泡和卵母细胞发育的影响.动物学研究,23(5),372-378. |
| MLA | 苏雷,et al."年龄和FSH在肉牛活体取卵过程中对卵泡和卵母细胞发育的影响".动物学研究 23.5(2002):372-378. |
入库方式： OAI收割
来源：昆明动物研究所
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