Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization
文献类型：期刊论文
| 作者 | Si W1,3; Zheng P1,3; Tang XH1,3; He XC1,3; Wang H1,3; Bavister BD2,3; Ji WZ[*]1,3 |
| 刊名 | CRYOBIOLOGY
 |
| 出版日期 | 2000 |
| 卷号 | 41期号:3页码:232-240 |
| 关键词 | cryopreservation Macaca mulatta spermatozoa cryoprotectants n vitro fertilization embryo development sugar glycerol semen capacitation plasma membrane |
| ISSN号 | 0011-2240 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Although spermatozoa from several species of nonhuman primates have been cryopreserved, there has been no report of success with rhesus macaque spermatozoa as judged by functional assays. Two Tris--egg yolk freezing media, TEST and TTE, which have been successfully used for cynomolgus macaque (Macaca fascicularis) spermatozoa, were compared for cryopreservation of spermatozoa from four rhesus macaques (Macaca mulatta). The postthaw motility (percentage and duration) of spermatozoa cryopreserved in TTE was much higher than that for spermatozoa cryopreserved in TEST. The function of sperm cryopreserved in TTE was evaluated by in vitro fertilization of oocytes collected from gonadotropin-stimulated prepubertal rhesus macaques. Of the inseminated oocytes, 82 +/- 13% were fertilized and 63 +/- 22 and 39 +/- 21% of the resulting zygotes developed into morulae and blastocysts, respectively. These results indicate that rhesus macaque spermatozoa can be effectively cryopreserved in TTE medium. This finding will facilitate the application of in vivo and in vitro assisted reproductive technologies in this species. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the China Na- tional Science Foundation (39970117), the Chinese Acad- emy of Sciences (KZ952-J1-109), the Yunnan Nature Sci- ences Foundation (98C092M), and the Fogarty International Center, National Institutes of Health, U.S.A. (TW00840). |
| 原文出处 | 200041232.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5535]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_哺乳动物胚胎发育 |
| 作者单位 | 1.Kunming Institute of Zoology, The Chinese Academy of Science, Kunming, Yunnan 650223, People’s Republic of China 2.Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, Wisconsin 53706, U.S.A. 3.The China–U.S. Primate Biology Laboratory, Kunming Institute of Zoology and University of Wisconsin-Madison |
| 推荐引用方式 GB/T 7714 | Si W,Zheng P,Tang XH,et al. Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization[J]. CRYOBIOLOGY,2000,41(3):232-240. |
| APA | Si W.,Zheng P.,Tang XH.,He XC.,Wang H.,...&Ji WZ[*].(2000).Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization.CRYOBIOLOGY,41(3),232-240. |
| MLA | Si W,et al."Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization".CRYOBIOLOGY 41.3(2000):232-240. |
入库方式： OAI收割
来源：昆明动物研究所
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