中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Amino acid requirements for maturation of rhesus monkey (Macacca mulatta) oocytes in culture

文献类型:期刊论文

作者Zheng P1,3; Bavister BD2,3; Ji WZ[*]1,3
刊名REPRODUCTION
出版日期2002
卷号124期号:4页码:515-522
ISSN号1470-1626
通讯作者wji@mail.kiz.ac.cn
合作状况其它
英文摘要This study evaluated the effects of different amino acid formulations on supporting meiotic and cytoplasmic maturation of rhesus monkey (Macacca mulatta) oocytes in vitro. Five hundred and forty-six cumulus-oocyte complexes (COCs) aspirated from unstimulated adult monkey follicles (greater than or equal to 1000 mum in diameter) were cultured in either modified Connaught Medical Research Laboratories 1066 medium (mCMRL-1066) or in one of eight chemically defined media (modified basic medium 5 supplemented with 5.5 mmol glucose l(-1), 0.003 mmol pantothenic acid l(-1) and different amino acid formulations) as below: (1) modified basic medium 5 (mBM5) containing no amino acid; (2) mBM5 + 0.2 mmol glutamine l(-1); (3) mBM5 + 11 amino acids from hamster embryo culture medium 6 (HECM-6) (11 AA); (4) mBM5 + Eagle's non-essential amino acids (NEA); (5) mBM5 + NEA + 0.2 mmol glutamine l(-1); (6) mBM5 + Eagle's essential amino acids (EA) without glutamine; (7) mBM5 + EA + 0.2 mmol glutamine l(-1); (8) mBM5 + Eagle's 20 amino acids (20 AA) + 0.2 mmol glutamine l(-1); and (9) mCMRL-1066 (control). All media contained FSH, LH, oestradiol and progesterone. After maturation, mature oocytes were subjected to the same fertilization and embryo culture procedures. COCs matured in treatment 5 had greater potential to progress to metaphase II (66%; P < 0.05) than did those in treatments 1 (37.3%), 2 (48.3%)f 3 (41%), 6 (41%) and 9 (43%). Oocytes matured in treatment 8 had the best morula (53%) and blastocyst (18%) developmental responses (P<0.05). The lowest (P<0.05) morula and blastocyst developmental responses were obtained from COCs matured in treatments 1 (0%) and 6 (8%). The other media supported intermediate embryonic development (range 11-38% of morula and blastocyst). These results indicate that the choice of amino acids affects the competence of oocyte maturation and that Eagle's 20 AA with 0.2 mmol glutamine l(-1) is more efficient than the other amino acid formulations for maturation of rhesus monkey oocytes.
收录类别SCI
资助信息This research was supported by the Major State Research Development Program G2000016108 and the key project of the Chinese Academy of Sciences, KSCX 1-05-01.
原文出处2002124515.pdf
语种英语
公开日期2010-08-24
源URL[http://159.226.149.42:8088/handle/152453/5553]  
专题昆明动物研究所_生殖与发育生物学
昆明动物研究所_哺乳动物胚胎发育
作者单位1.Chinese Acad Sci, Dept Reprod Biol, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China
2.Audubon Inst Ctr Res Endangered Species, New Orleans, LA 70131 USA
3.Chinese Acad Sci, Kunming Inst Zool, China US Primate Biol Lab, Kunming 650223, Yunnan, Peoples R China
推荐引用方式
GB/T 7714
Zheng P,Bavister BD,Ji WZ[*]. Amino acid requirements for maturation of rhesus monkey (Macacca mulatta) oocytes in culture[J]. REPRODUCTION,2002,124(4):515-522.
APA Zheng P,Bavister BD,&Ji WZ[*].(2002).Amino acid requirements for maturation of rhesus monkey (Macacca mulatta) oocytes in culture.REPRODUCTION,124(4),515-522.
MLA Zheng P,et al."Amino acid requirements for maturation of rhesus monkey (Macacca mulatta) oocytes in culture".REPRODUCTION 124.4(2002):515-522.

入库方式: OAI收割

来源:昆明动物研究所

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