Amino acid requirements for maturation of rhesus monkey (Macacca mulatta) oocytes in culture
文献类型：期刊论文
| 作者 | Zheng P1,3; Bavister BD2,3; Ji WZ[*]1,3 |
| 刊名 | REPRODUCTION
 |
| 出版日期 | 2002 |
| 卷号 | 124期号:4页码:515-522 |
| ISSN号 | 1470-1626 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | This study evaluated the effects of different amino acid formulations on supporting meiotic and cytoplasmic maturation of rhesus monkey (Macacca mulatta) oocytes in vitro. Five hundred and forty-six cumulus-oocyte complexes (COCs) aspirated from unstimulated adult monkey follicles (greater than or equal to 1000 mum in diameter) were cultured in either modified Connaught Medical Research Laboratories 1066 medium (mCMRL-1066) or in one of eight chemically defined media (modified basic medium 5 supplemented with 5.5 mmol glucose l(-1), 0.003 mmol pantothenic acid l(-1) and different amino acid formulations) as below: (1) modified basic medium 5 (mBM5) containing no amino acid; (2) mBM5 + 0.2 mmol glutamine l(-1); (3) mBM5 + 11 amino acids from hamster embryo culture medium 6 (HECM-6) (11 AA); (4) mBM5 + Eagle's non-essential amino acids (NEA); (5) mBM5 + NEA + 0.2 mmol glutamine l(-1); (6) mBM5 + Eagle's essential amino acids (EA) without glutamine; (7) mBM5 + EA + 0.2 mmol glutamine l(-1); (8) mBM5 + Eagle's 20 amino acids (20 AA) + 0.2 mmol glutamine l(-1); and (9) mCMRL-1066 (control). All media contained FSH, LH, oestradiol and progesterone. After maturation, mature oocytes were subjected to the same fertilization and embryo culture procedures. COCs matured in treatment 5 had greater potential to progress to metaphase II (66%; P < 0.05) than did those in treatments 1 (37.3%), 2 (48.3%)f 3 (41%), 6 (41%) and 9 (43%). Oocytes matured in treatment 8 had the best morula (53%) and blastocyst (18%) developmental responses (P<0.05). The lowest (P<0.05) morula and blastocyst developmental responses were obtained from COCs matured in treatments 1 (0%) and 6 (8%). The other media supported intermediate embryonic development (range 11-38% of morula and blastocyst). These results indicate that the choice of amino acids affects the competence of oocyte maturation and that Eagle's 20 AA with 0.2 mmol glutamine l(-1) is more efficient than the other amino acid formulations for maturation of rhesus monkey oocytes. |
| 收录类别 | SCI |
| 资助信息 | This research was supported by the Major State Research Development Program G2000016108 and the key project of the Chinese Academy of Sciences, KSCX 1-05-01. |
| 原文出处 | 2002124515.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5553]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_哺乳动物胚胎发育 |
| 作者单位 | 1.Chinese Acad Sci, Dept Reprod Biol, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China 2.Audubon Inst Ctr Res Endangered Species, New Orleans, LA 70131 USA 3.Chinese Acad Sci, Kunming Inst Zool, China US Primate Biol Lab, Kunming 650223, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng P,Bavister BD,Ji WZ[*]. Amino acid requirements for maturation of rhesus monkey (Macacca mulatta) oocytes in culture[J]. REPRODUCTION,2002,124(4):515-522. |
| APA | Zheng P,Bavister BD,&Ji WZ[*].(2002).Amino acid requirements for maturation of rhesus monkey (Macacca mulatta) oocytes in culture.REPRODUCTION,124(4),515-522. |
| MLA | Zheng P,et al."Amino acid requirements for maturation of rhesus monkey (Macacca mulatta) oocytes in culture".REPRODUCTION 124.4(2002):515-522. |
入库方式： OAI收割
来源：昆明动物研究所
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