Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm
文献类型：期刊论文
| 作者 | Si W1,2; Zheng P1; Li YH1,3; Dinnyes A4,5; Ji WZ[*]1 |
| 刊名 | AMERICAN JOURNAL OF PRIMATOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 62期号:4页码:301-306 |
| 关键词 | cryopreservation rhesus monkey spermatozoa glycerol DMSO |
| ISSN号 | 0275-2565 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Glycerol and dimethyl sulfoxide (DMSO) are widely used as penetrating cryoprotectants in the freezing of sperm, and various concentrations are applied in different species and laboratories. The present study aimed to examine the effect of these two cryoprotectants at different concentrations (2%, 5%, 10%, and 15% glycerol or DMSO) on rhesus monkey sperm cryopreservation. The results showed that the highest recovery of post-thaw sperm motility, and plasma membrane and acrosome integrity was achieved when the sperm was frozen with 5% glycerol. Spermatozoa cryopreserved with 15% DMSO showed the lowest post-thaw sperm motility, and spermatozoa cryopreserved with 15% glycerol and 15% DMSO showed the lowest plasma membrane integrity among the eight groups. The results achieved with 5% glycerol were significantly better for all parameters than those obtained with 5% DMSO. The functional cryosurvival of sperm frozen with 5% glycerol was further assessed by in vitro fertilization (IVF). Overall, 85.7% of the oocytes were successfully fertilized, and 51.4% and 5.7% of the resulting zygotes developed into morulae and blastocysts, respectively. The results indicate that the type and concentration of the penetrating cryoprotectant used can greatly affect the survival of rhesus monkey sperm after it is frozen and thawed. The suitable glycerol level for rhesus monkey sperm freezing is 5%, and DMSO is not suitable for rhesus monkey sperm cryopreservation. |
| 收录类别 | SCI |
| 资助信息 | Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009–09; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Techno- logical Collaboration Project; Contract grant number: TET CHN14/02. |
| 原文出处 | 200462301.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5573]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_哺乳动物胚胎发育 |
| 作者单位 | 1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, People’s Republic of China 2.Graduate School of the Chinese Academy of Sciences, Beijing, People’s Republic of China 3.College of Food Science and Technology, Yunnan Agricultural University, Kunming, People’s Republic of China 4.Research Group for Applied Animal Genetics and Biotechnology, Hungarian Academy of Sciences and Szent Istvan University, Godollo, Hungary 5.Department of Animal Biology, Agricultural Biotechnology Center, Godollo, Hungary |
| 推荐引用方式 GB/T 7714 | Si W,Zheng P,Li YH,et al. Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm[J]. AMERICAN JOURNAL OF PRIMATOLOGY,2004,62(4):301-306. |
| APA | Si W,Zheng P,Li YH,Dinnyes A,&Ji WZ[*].(2004).Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm.AMERICAN JOURNAL OF PRIMATOLOGY,62(4),301-306. |
| MLA | Si W,et al."Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm".AMERICAN JOURNAL OF PRIMATOLOGY 62.4(2004):301-306. |
入库方式： OAI收割
来源：昆明动物研究所
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