中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 昆明动物研究所
  3. 昆明动物研究所
  4. 生殖与发育生物学
Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender

文献类型：期刊论文

作者Li YH1,2,3; Cai KJ1; Su L1; Guan M1; He XC1; Wang H1; Kovacs A4; Ji WZ[*]1
刊名ASIAN JOURNAL OF ANDROLOGY
出版日期2005
卷号7期号:2页码:139-144
关键词cryopreservation Macaca fascicularis chemically defined extender glycerol
ISSN号1008-682X
通讯作者wji@mail.kiz.ac.cn
合作状况其它
英文摘要AIM: To establish a method for cynomolgus monkey sperm cryopreservation in a chemically defined extender. METHODS: Semen samples were collected by electro-ejaculation from four sexually mature male cynomolgus monkeys. The spermatozoa were frozen in straws by liquid nitrogen vapor using egg-yolk-free Tes-Tris mTTE synthetic extender and glycerol as cryoprotectant. The effects of glycerol concentration (1 %, 3 %, 5 %, 10 % and 15 % [v/v]) and its equilibration time (10 min, 30 min, 60 min and 90 min) on post-thaw spermatozoa were examined by sperm motility and sperm head membrane integrity. RESULTS: The post-thaw motility and head membrane integrity of spermatozoa were significantly higher (P0.05) for 5 % glycerol (42.95 +/- 2.55 and 50.39+/- 2.42, respectively) than those of the other groups (1%: 19.19 +/- 3.22 and 24.84 +/- 3.64; 3%: 34.23 +/- 3.43 and 41.37 +/- 3.42; 10%:15.68 +/- 2.36 and 21.39 +/- 3.14; 15%: 7.47 +/- 1.44 and 12.90 +/- 2.18). The parameters for 30 min equilibration(42.95 +/- 2.55 and 50.39 +/- 2.42) were better (P0.05) than those of the other groups (10 min: 31.33 +/- 3.06 and 38.98 +/- 3.31; 60 min: 32.49 +/- 3.86 and 40.01 +/- 4.18; 90 min: 31.16 +/- 3.66 and 38.30 +/- 3.78). Five percent glycerol and 30 min equilibration yielded the highest post-thaw sperm motility and head membrane integrity. CONCLUSION: Cynomolgus monkey spermatozoa can be successfully cryopreserved in a chemically defined extender, which is related to the concentration and the equilibration time of glycerol.
收录类别SCI
资助信息This work was supported by grants from the Chinese Academy of Science’s KSCX 1-05-01, major state research development program G2000016108, Ministry of Science and Technology of China (2001 DEA 10009-09) and National Nature Sci- ence Foundation of China (No. 30370166).
原文出处20057139.pdf
语种英语
公开日期2010-08-24
源URL[http://159.226.149.42:8088/handle/152453/5587]  
专题昆明动物研究所_生殖与发育生物学
昆明动物研究所_哺乳动物胚胎发育
作者单位1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
3.College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650201, China
4.Research Institute for Animal Breeding and Nutrition, 2053 Herceghalom, Hungary
推荐引用方式
GB/T 7714		 Li YH,Cai KJ,Su L,et al. Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender[J]. ASIAN JOURNAL OF ANDROLOGY,2005,7(2):139-144.
APA Li YH.,Cai KJ.,Su L.,Guan M.,He XC.,...&Ji WZ[*].(2005).Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender.ASIAN JOURNAL OF ANDROLOGY,7(2),139-144.
MLA Li YH,et al."Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender".ASIAN JOURNAL OF ANDROLOGY 7.2(2005):139-144.

入库方式： OAI收割

来源：昆明动物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。