Superovulatory response to a low dose single-daily treatment of rhFSH dissolved in polyvinylpyrrolidone in rhesus monkeys
文献类型：期刊论文
| 作者 | Yang SH1; He XC1; Hildebrandt TB2; Zhou Q3; Ji WZ[*]1 |
| 刊名 | AMERICAN JOURNAL OF PRIMATOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 69期号:11页码:1278-1284 |
| 关键词 | rhFSH polyvinylpyrrolidone ovarian stimulation rhesus monkey |
| ISSN号 | 0275-2565 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 英文摘要 | To simplify the procedure for superovulation in the rhesus monkey, this study was designed using polyvinylpyrrolidone (PVP) solution as a solvent for gonadotropins. Thirty-five cycling females (aged 5-8 years old) were divided into six groups during the breeding season (November-February). The groups were as follows: Group I, animals received twice-daily 35 IU recombinant human follicle-stimulating hormone (rhFSH) dissolved in 0.5 ml saline for 8 days as the control; Groups II and III, animals received single-daily 35 IU and 17 IU rhFSH in 0.5 saline, respectively, for 9 days; Groups IV, V and VIE received single-daily injection of 35 IU rhFSH, 17 IU rhFSH and 8.5 IU rhFSH dissolved in 0.5 ml 30% PVP (w/v) solution, respectively, for 9 days. After human chorionic gonadotropin was administered to induce the nuclear maturation of oocytes, oocytes were retrieved and the development competence of recovered oocytes treated with in vitro fertilization were observed. The plasma concentrations of follicle-stimulating hormone and ovarian responses were monitored during the treatment. The results showed that the number of recovered oocytes and the in vitro developmental competence of mature oocytes was equivalent among monkeys when treated with a single-daily treatment of 17 and 35 IU rhFSH with PVP preparation in Groups IV and V compared with the twice-daily 35 IU rhFSH treatments received by Group I. However, almost all animals in Groups II, III and VI responded poorly to corresponding stimulations. These findings indicate that a single-daily low dose of rhFSH dissolved in PVP solution can induce the satisfactory ovarian responses in rhesus monkeys. This has the potential to reduce treatment distress, stress to the animals, the labor of the operator as well as the amount of rhFSH used in ovarian stimulation, compared with traditional superovulation methods. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China; Contract grant number: 30370166; Contract grant sponsor: Joint Scholar Plan for the Development of Western China and KSCX1-YW-R-47 from the Chinese Academy of Sciences; Contract grant sponsor: National Application Research Program of China (863 program); Contract grant number: 2005AA219010; Contract grant sponsor: German Max-Planck-Society and Chinese Academy of Sciences. |
| 原文出处 | 2007691278.pdf |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5607]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academyof Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming,Yunnan, P.R. China 2.Institute for Zoo and Wildlife Research, Berlin, Germany 3.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academyof Sciences, Beijing, P.R. China |
| 推荐引用方式 GB/T 7714 | Yang SH,He XC,Hildebrandt TB,et al. Superovulatory response to a low dose single-daily treatment of rhFSH dissolved in polyvinylpyrrolidone in rhesus monkeys[J]. AMERICAN JOURNAL OF PRIMATOLOGY,2007,69(11):1278-1284. |
| APA | Yang SH,He XC,Hildebrandt TB,Zhou Q,&Ji WZ[*].(2007).Superovulatory response to a low dose single-daily treatment of rhFSH dissolved in polyvinylpyrrolidone in rhesus monkeys.AMERICAN JOURNAL OF PRIMATOLOGY,69(11),1278-1284. |
| MLA | Yang SH,et al."Superovulatory response to a low dose single-daily treatment of rhFSH dissolved in polyvinylpyrrolidone in rhesus monkeys".AMERICAN JOURNAL OF PRIMATOLOGY 69.11(2007):1278-1284. |
入库方式： OAI收割
来源：昆明动物研究所
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