Epigenetic marks in cloned rhesus monkey embryos: Comparison with counterparts produced in vitro
文献类型：期刊论文
| 作者 | Yang JF4,5; Yang SH4; Beaujean N6,7; Niu YY4; He XC4; Xie YH4; Tang XH4; Wang L7; Zhou Q[*]7; Ji WZ[*]4 |
| 刊名 | BIOLOGY OF REPRODUCTION
 |
| 出版日期 | 2007 |
| 卷号 | 76期号:1页码:36-42 |
| 关键词 | DNA methylation donor cells early development embryo |
| ISSN号 | 0006-3363 |
| 通讯作者 | qzhou@ioz.ac.cn ; wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Until now, no primate animals have been successfully cloned to birth with somatic cell nuclear transfer (SCNT) procedures, and little is known about the molecular events that occurred in the reconstructed embryos during preimplantation development. In many SCNT cases, epigenetic reprogramming of the donor nuclei after transfer into enucleated oocytes was hypothesized to be crucial to the reestablishment of embryonic totipotency. In the present study, we focused on two major epigenetic marks, DNA methylation and histone H3 lysine 9 (H3K9) acetylation, which we examined by indirect immunofluorescence and confocal laser scanning microscopy. During preimplantation development, 67% of two-cell- and 50% of eight-cell-cloned embryos showed higher DNA methylation levels than their in vitro fertilization (IVF) counterparts, which undergo gradual demethylation until the early morula stage. Moreover, whereas an asymmetric distribution of DNA methylation was established in an IVF blastocysts with a lower methylation level in the inner cell mass (ICM) than in the trophectoderm, in most cloned blastocysts, ICM cells maintained a high degree of methylation. Finally, two donor cell lines (S11 and S1-04) that showed a higher level of H3K9 acetylation supported more blastocyst formation after nuclear transfer than the other cell line (S1-03), with a relatively low level of acetylation staining. In conclusion, we propose that abnormal DNA methylation patterns contribute to the poor quality of cloned preimplantation embryos and may be one of the obstacles to successful cloning in primates. |
| 收录类别 | SCI |
| 资助信息 | Supported by Major Projects of Knowledge Innovation Program from the Chinese Academy of Sciences (KSCX1-05), National Natural Science Foundation of China (30370166), Joint Scholar Plan for the Development of Western China, National Basic Research Program of China (973 program, 2004CCA01300), and the PRA (Programme de Recherches Avance´es Franco—Chinois). |
| 原文出处 | 20077636.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5619]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.** 2.** 3.*** 4.Department of Reproduction and Development,Kunming Institute of Zoology & Kunming Primate Research Center, the Chinese Academy of Sciences, Kunming, Yunnan 650223, China 5.Graduate University of Chinese Academy of Sciences,Beijing 100049, China 6.INRA,UMR 1198; ENVA; CNRS, FRE 2857, Biologie du De ´veloppement et Reproduction, Jouy en Josas, F-78350, France 7.The State Key Laboratory of Reproductive Biology,Institute of Zoology, the Chinese Academy of Sciences, Beijing 100860, China |
| 推荐引用方式 GB/T 7714 | Yang JF,Yang SH,Beaujean N,et al. Epigenetic marks in cloned rhesus monkey embryos: Comparison with counterparts produced in vitro[J]. BIOLOGY OF REPRODUCTION,2007,76(1):36-42. |
| APA | Yang JF.,Yang SH.,Beaujean N.,Niu YY.,He XC.,...&Ji WZ[*].(2007).Epigenetic marks in cloned rhesus monkey embryos: Comparison with counterparts produced in vitro.BIOLOGY OF REPRODUCTION,76(1),36-42. |
| MLA | Yang JF,et al."Epigenetic marks in cloned rhesus monkey embryos: Comparison with counterparts produced in vitro".BIOLOGY OF REPRODUCTION 76.1(2007):36-42. |
入库方式： OAI收割
来源：昆明动物研究所
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