Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector
文献类型：期刊论文
| 作者 | Niu YY1,2,3,4; Yu Y5; Bernat A6,7,8; Yang SH1,2; He XC1,2,3,4; Guo XY1,2,3,4; Chen DL1,2,3,4; Chen YC1,2,3,4; Ji SH1,2,3; Si W1,2,3,4 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2010 |
| 卷号 | 107期号:41页码:17663-17667 |
| 关键词 | lentiviral vector transgenesis |
| 通讯作者 | pierre.savatier@inserm.fr ; kangzhang@gmail.com ; qzhou@ioz.ac.cn ; wji@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The development of transgenic technologies in monkeys is important for creating valuable animal models of human physiology so that the etiology of diseases can be studied and potential therapies for their amelioration may be developed. However, the efficiency of producing transgenic primate animals is presently very low, and there are few reports of success. We have developed an improved methodology for the production of transgenic rhesus monkeys, making use of a simian immunodeficiency virus (SIV)-based vector that encodes EGFP and a protocol for infection of early-cleavage-stage embryos. We show that infection does not alter embryo development. Moreover, the timing of infection, either before or during embryonic genome activation, has no observable effect on the level and stability of transgene expression. Of 70 embryos injected with concentrated virus at the one-to two-cell stage or the four-to eight-cell stage and showing fluorescence, 30 were transferred to surrogate mothers. One transgenic fetus was obtained from a fraternal triple pregnancy. Four infant monkeys were produced from four singleton pregnancies, of which two expressed EGFP throughout the whole body. These results demonstrate the usefulness of SIV-based lentiviral vectors for the generation of transgenic monkeys and improve the efficiency of transgenic technology in nonhuman primates. |
| 收录类别 | SCI |
| 资助信息 | W.J. was supported by Grants KSCX1-05-02 and KSCX2-YW-R-47from the Chinese Academy of Sciences; 2006CB701505 and 2007CB947701 from the Major State Basic Development Program; 2006AA02A116 and 2006AA02A101 from the National High Technology Research and Development Program; 2007BAI33B00 from the National Key Technology R&D Program; 30701059 and 30700425 from the China Na- tional Science Foundation; 2006PT08-2 from the R&D Infrastructure and Facility Development Program of Yunnan Province; 2007GH from the So- cial Science and Technology Development Program of Yunnan Province; and 2009ZX09501-028 from the National Science and Technology Major Project. K.Z. is supported by the Chinese National 985 Project to Sichuan University, by the US National Eye Institute/National Institutes of Health, by a Veterans Administration Merit Award, Research to Prevent Blindness, and by a Burroughs Wellcome Fund Clinical Scientist Award in Transla- tional Research. |
| 语种 | 英语 |
| 公开日期 | 2010-11-01 |
| 源URL | [http://159.226.149.42:8088/handle/152453/6340]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Kunming Primate Research Center, Kunming Institute of Zoology 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, People’s Republic of China 3.Kunming Biomed International 4.National Engineering Research Center of Biomedicine and Animal Science, Kunming 650051, People’s Republic of Chin 5.Institute of Zoology, Chinese Academy of Sciences, Beijing 100860, People’s Republic of China 6.Institut National de la Santé et de la Recherche Médicale, U846 7.PrimaStem Stem Cell and Brain Research Institute, Bron 69500, France 8.Université de Lyon, Lyon 69003, France 9.Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650093, People’s Republic of China 10.Center for Molecular Medicine and Department of Ophthalmology, West China Hospital |
| 推荐引用方式 GB/T 7714 | Niu YY,Yu Y,Bernat A,et al. Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2010,107(41):17663-17667. |
| APA | Niu YY.,Yu Y.,Bernat A.,Yang SH.,He XC.,...&Savatier P[*].(2010).Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,107(41),17663-17667. |
| MLA | Niu YY,et al."Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.41(2010):17663-17667. |
入库方式： OAI收割
来源：昆明动物研究所
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