Optimization of Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Cryopreservation of Rhesus Macaque (Macaca mulatta) Sperm
文献类型：期刊论文
| 作者 | Yang SH1,2; Ping SH1,2; Si W[*]2; He XC2; Wang XY2; Lu YQ2; Ji SH2; Niu YY2; Ji WZ2,3 |
| 刊名 | JOURNAL OF VETERINARY MEDICAL SCIENCE
 |
| 出版日期 | 2011 |
| 卷号 | 73期号:6页码:717-723 |
| 关键词 | cryopreservation freezing of semen in vitro fertilization rhesus macaque |
| 通讯作者 | siw@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Ethylene glycol (EG) has been speculated to be the most appropriate penetrating cryoprotectant for cryopreservation of rhesus macaque sperm due to its higher permeability coefficient. The present study aimed to determine the optimal EG concentration, freezing rate and holding time in liquid nitrogen (LN(2)) vapor for rhesus sperm cryopreservation. Among six tested EG concentrations (0, 0.18, 0.35, 0.7, 1.4 and 2.1 M), 0.7 M EG showed the most effective cryoprotection (P<0.05). Sperm frozen with 0.7 M EG at -183 degrees C/min showed higher post-thaw motility than sperm frozen at -10, -67 or -435 degrees C/min (P<0.05). Sperm frozen in LN(2) vapor at -183 degrees C/min with 0.7 M EG and a holding time of 10 min showed higher post-thaw motility compared with a holding time of 5 or 15 min (P<0.05). The function of sperm cryopreserved at the optimized EG concentration, freezing rate and holding time was further evaluated by in vitro fertilization. Of the 36 oocytes collected from gonadotropin-stimulated rhesus macaques, 61.1% were fertilized, and 61.1, 44.4 and 36.1% of the oocytes developed to 2 cells, morulae and blastocysts, respectively. Our findings provide an alternative penetrating cryoprotectant and optimal protocol for genetic preservation purposes in this important species. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by research grants from the National Key Technologies R & D Program of China (2007BAI33B00), the Chinese Major State Basic Development Program (2006CB701505 and 2007CB947701) and the Chinese National Science Founda- tion (31071279 and 30871232). |
| 语种 | 英语 |
| 公开日期 | 2011-08-09 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6630]  |
| 专题 | 昆明动物研究所_生殖与发育生物学 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Laboratory of Moleculac Genetics of Aging and Tumor, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224 2.Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming 650223 3.Kunming Biomed International, National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, P.R. China |
| 推荐引用方式 GB/T 7714 | Yang SH,Ping SH,Si W[*],et al. Optimization of Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Cryopreservation of Rhesus Macaque (Macaca mulatta) Sperm[J]. JOURNAL OF VETERINARY MEDICAL SCIENCE,2011,73(6):717-723. |
| APA | Yang SH.,Ping SH.,Si W[*].,He XC.,Wang XY.,...&Ji WZ.(2011).Optimization of Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Cryopreservation of Rhesus Macaque (Macaca mulatta) Sperm.JOURNAL OF VETERINARY MEDICAL SCIENCE,73(6),717-723. |
| MLA | Yang SH,et al."Optimization of Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Cryopreservation of Rhesus Macaque (Macaca mulatta) Sperm".JOURNAL OF VETERINARY MEDICAL SCIENCE 73.6(2011):717-723. |
入库方式： OAI收割
来源：昆明动物研究所
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