PtdIns(3,4,5)P-3 is constitutively synthesized and required for spindle translocation during meiosis in mouse oocytes
文献类型：期刊论文
| 作者 | Zheng P[*]1,2; Baibakov B3; Wang XH2; Dean J[*]3 |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2013 |
| 卷号 | 126期号:3页码:715-721 |
| 关键词 | PtdIns(3,4,5)P-3 Oocyte meiosis Spindle translocation Filamentous actin Cdc42 |
| 通讯作者 | zhengp@mail.kiz.ac.cn ; jurrien@helix.nih.gov |
| 合作状况 | 其它 |
| 英文摘要 | Prior to ovulation, mammalian oocytes complete their first meiotic division and arrest at metaphase II. During this marked asymmetric cell division, the meiotic spindle moves dramatically from the center of the oocyte to the cortex to facilitate segregation of half of its chromosomal content into the diminutive first polar body. Recent investigations have documented crucial roles for filamentous actin (F-actin) in meiotic spindle translocation. However, the identity of the upstream regulators responsible for these carefully orchestrated movements has remained elusive. Utilizing fluorescently tagged probes and time-lapse confocal microscopy, we document that phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P-3] is constitutively synthesized with spatial and temporal dynamics similar to that of F-actin and Formin 2 (Fmn2). Blockage of PtdIns(3,4,5) P-3 synthesis by LY294002, a specific inhibitor of phosphoinositide 3-kinase (PI3K), disrupts cytoplasmic F-actin organization and meiotic spindle migration to the cortex. F-actin nucleator Fmn2 and Rho GTPase Cdc42 play roles in mediating the effect of PtdIns(3,4,5)P-3 on F-actin assembly. Moreover, the spatial and temporal dynamics of PtdIns(3,4,5)P-3 is impaired by depletion of MATER or Filia, two oocyte proteins encoded by maternal effect genes. Thus, PtdIns(3,4,5)P-3 is synthesized during meiotic maturation and acts upstream of Cdc42 and Fmn2, but downstream of MATER/Filia proteins to regulate the F-actin organization and spindle translocation to the cortex during mouse oocyte meiosis. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, USA (to J. D.); and the Natural Science Foundation of China [grant number 31071274 to P. Zheng]. |
| 语种 | 英语 |
| WOS记录号 | WOS:000317281700002 |
| 公开日期 | 2013-05-17 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7431]  |
| 专题 | 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 3.Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA |
| 推荐引用方式 GB/T 7714 | Zheng P[*],Baibakov B,Wang XH,et al. PtdIns(3,4,5)P-3 is constitutively synthesized and required for spindle translocation during meiosis in mouse oocytes[J]. JOURNAL OF CELL SCIENCE,2013,126(3):715-721. |
| APA | Zheng P[*],Baibakov B,Wang XH,&Dean J[*].(2013).PtdIns(3,4,5)P-3 is constitutively synthesized and required for spindle translocation during meiosis in mouse oocytes.JOURNAL OF CELL SCIENCE,126(3),715-721. |
| MLA | Zheng P[*],et al."PtdIns(3,4,5)P-3 is constitutively synthesized and required for spindle translocation during meiosis in mouse oocytes".JOURNAL OF CELL SCIENCE 126.3(2013):715-721. |
入库方式： OAI收割
来源：昆明动物研究所
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